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RecombinantRabMAb

Recombinant Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)

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Western blot - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
  • Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
  • Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13949] to ADH7 - BSA and Azide free
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-ADH7 antibody [EPR13949] - BSA and Azide free
    See all ADH7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13949] to ADH7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250872 is the carrier-free version of ab186408.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab250872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Notes
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Target

  • Relevance

    This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members.

  • Cellular localization

    Cytoplasm

  • Database links

    • Alternative names

      • ADH 4 antibody
      • ADH 7 antibody
      • ADH, class IV antibody
      • ADH4 antibody
      • Alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide antibody
      • alcohol dehydrogenase 7 antibody
      • alcohol dehydrogenase class 4 mu/sigma chain antibody
      • Alcohol dehydrogenase class IV mu/sigma chain antibody
      • alcohol dehydrogenase VII antibody
      • class IV sigma 1 alcohol dehydrogenase antibody
      • class IV sigmasigma alcohol dehydrogenase antibody
      • Gastric alcohol dehydrogenase antibody
      • Gastric H(+)/K(+) ATPase subunit alpha antibody
      • Retinol dehydrogenase antibody
      see all

    Images

    • Western blot - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
      Western blot - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
      All lanes : Anti-ADH7 antibody [EPR13949] (ab186408) at 1/1000 dilution

      Lane 1 : Human stomach lysate
      Lane 2 : Human fetal liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa



      This data was developed using ab186408, the same antibody clone in a different buffer formulation.

    • Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
      Immunocytochemistry/ Immunofluorescence - Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
      This data was developed using ab186408, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling ADH7 with ab186408 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
    • Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)
      Anti-ADH7 antibody [EPR13949] - BSA and Azide free (ab250872)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250872? Please let us know so that we can cite the reference in this datasheet.

    ab250872 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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