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RecombinantRabMAb

Recombinant Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)

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  • Certificate of Compliance
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Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
  • Western blot - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
  • Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12501] to ADHFE1 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

You may also be interested in

Conjugation
Product image
HRP Conjugation Kit - Lightning-Link® (ab102890)
Primary
Product image
Anti-ADHFE1 antibody [EPR12501] (ab173530)

View more associated products

Overview

  • Product name

    Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free
    See all ADHFE1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12501] to ADHFE1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human ADHFE1. The exact sequence is proprietary.
    Database link: Q8IWW8

  • General notes

    ab249768 is the carrier-free version of ab173530.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12501
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Alternative Versions

    • Anti-ADHFE1 antibody [EPR12501] (ab173530)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab249768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
IP
Use at an assay dependent concentration.
Application notes
Is unsuitable for ICC/IF or IHC-P.

Target

  • Target information above from: UniProt accession Q8IWW8 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 137872 Human
    • Omim: 611083 Human
    • SwissProt: Q8IWW8 Human
    • Unigene: 720023 Human
    • Alternative names

      • ADH8 antibody
      • ADHFE 1 antibody
      • ADHFe1 antibody
      • Alcohol dehydrogenase iron-containing protein 1 antibody
      • Alcohol dehydrogenase, iron containing, 1 antibody
      • Fe containing alcohol dehydrogenase antibody
      • Fe-containing alcohol dehydrogenase antibody
      • HOT antibody
      • HOT_HUMAN antibody
      • Hydroxyacid oxoacid transhydrogenase, mitochondrial antibody
      • Hydroxyacid-oxoacid transhydrogenase antibody
      • mitochondrial antibody
      see all

    Images

    • Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
      Immunoprecipitation - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
      This data was developed using ab173530, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver lysate or (Lane 2) 1X PBS (negative control) using ab173530 at a 1/10 dilution to immunoprecipitate ADHFE1, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
    • Western blot - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
      Western blot - Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
      All lanes : Anti-ADHFE1 antibody [EPR12501] (ab173530) at 1/1000 dilution

      Lane 1 : Human fetal liver lysates
      Lane 2 : Human fetal kidney lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 50 kDa



      This data was developed using ab173530, the same antibody clone in a different buffer formulation.

       

    • Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)
      Anti-ADHFE1 antibody [EPR12501] - BSA and Azide free (ab249768)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab249768? Please let us know so that we can cite the reference in this datasheet.

    ab249768 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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