Anti-AGE antibody (ab23722)
Key features and details
- Rabbit polyclonal to AGE
- Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-AGE antibody
See all AGE primary antibodies -
Description
Rabbit polyclonal to AGE -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length protein corresponding to Human AGE conjugated to bovine serum albumin. Native protein
(Peptide available asab129535) -
General notes
ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.15
Preservative: 0.05% Sodium azide
Constituents: 0.134% PBS, 0.85% Sodium chloride -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| WB | (2) |
Use at an assay dependent concentration.
|
| IHC-P | (3) |
Use at an assay dependent concentration. PubMed: 19223295
|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| ELISA |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. PubMed: 19223295 |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
ELISA
Use a concentration of 1 µg/ml. |
Target
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Relevance
The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure. -
Cellular localization
Cell Membrane and Secreted -
Alternative names
- Advanced glycation end products antibody
- AGEs antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (79)
ab23722 has been referenced in 79 publications.
- Mascolo E et al. Functional Inactivation of DrosophilaGCK Orthologs Causes Genomic Instability and Oxidative Stress in a Fly Model of MODY-2. Int J Mol Sci 22:N/A (2021). PubMed: 33477627
- Song S et al. Stimulation of AMPK Prevents Diabetes-Induced Photoreceptor Cell Degeneration. Oxid Med Cell Longev 2021:5587340 (2021). PubMed: 34093959
- MacDonald TL et al. Hyperglycaemia is associated with impaired muscle signalling and aerobic adaptation to exercise. Nat Metab 2:902-917 (2020). PubMed: 32694831
- Menini S et al. Diabetes promotes invasive pancreatic cancer by increasing systemic and tumour carbonyl stress in KrasG12D/+ mice. J Exp Clin Cancer Res 39:152 (2020). PubMed: 32778157
- Senatus L et al. RAGE impairs murine diabetic atherosclerosis regression and implicates IRF7 in macrophage inflammation and cholesterol metabolism. JCI Insight 5:N/A (2020). PubMed: 32641587