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    products/primary-antibodies/age-antibody-ab23722.pdf

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Immunology Immune System Diseases Autoimmune
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Anti-AGE antibody (ab23722)

  • Datasheet
  • SDS
Reviews (6)Q&A (22)References (101)

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Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)

    Key features and details

    • Rabbit polyclonal to AGE
    • Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, ELISA
    • Reacts with: Species independent
    • Isotype: IgG

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    Overview

    • Product name

      Anti-AGE antibody
      See all AGE primary antibodies
    • Description

      Rabbit polyclonal to AGE
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Fr, WB, IHC-P, ICC/IF, ELISAmore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Full length protein corresponding to Human AGE conjugated to bovine serum albumin. Native protein
      (Peptide available as ab129535)

    • General notes


      ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.15
      Preservative: 0.05% Sodium azide
      Constituents: 0.134% PBS, 0.85% Sodium chloride
    • Concentration information loading...
    • Purity

      Protein A purified
    • Primary antibody notes

      ab23722 is suitable for the detection of different AGE products in tissues, tissue extracts and body fluids.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Immune System Diseases
      • Autoimmune
      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • AGE-BSA (ab51995)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab23722 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-Fr (1)
    Use at an assay dependent concentration.
    WB (2)
    Use at an assay dependent concentration.
    IHC-P (3)
    Use at an assay dependent concentration. PubMed: 19223295
    ICC/IF
    Use a concentration of 5 µg/ml.
    ELISA
    Use a concentration of 1 µg/ml.
    Notes
    IHC-Fr
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration.
    IHC-P
    Use at an assay dependent concentration. PubMed: 19223295
    ICC/IF
    Use a concentration of 5 µg/ml.
    ELISA
    Use a concentration of 1 µg/ml.

    Target

    • Relevance

      The non enzymatic reaction of reducing carbohydrates with lysine side chains and N terminal amino groups of macromolecules (amino acids, proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The latter products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins, lipids and DNA. In long lived tissue proteins, these chemical modifications accumulate with age and may contribute to the pathophysiology of ageing and long term complications of diabetes, atherosclerosis and renal failure.
    • Cellular localization

      Cell Membrane and Secreted
    • Alternative names

      • Advanced glycation end products antibody
      • AGEs antibody

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
      Immunocytochemistry/ Immunofluorescence - Anti-AGE antibody (ab23722)
      ICC/IF image of ab23722 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab23722, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (101)

    Publishing research using ab23722? Please let us know so that we can cite the reference in this datasheet.

    ab23722 has been referenced in 101 publications.

    • López-Grueso MJ  et al. Deficiency of Parkinson's Related Protein DJ-1 Alters Cdk5 Signalling and Induces Neuronal Death by Aberrant Cell Cycle Re-entry. Cell Mol Neurobiol 43:757-769 (2023). PubMed: 35182267
    • Ren X  et al. Thioredoxin upregulation delays diabetes-induced photoreceptor cell degeneration via AMPK-mediated autophagy and exosome secretion. Diabetes Res Clin Pract 185:109788 (2022). PubMed: 35182712
    • Xia B  et al. Lycopene Improves Bone Quality and Regulates AGE/RAGE/NF-кB Signaling Pathway in High-Fat Diet-Induced Obese Mice. Oxid Med Cell Longev 2022:3697067 (2022). PubMed: 35222796
    • Xia S  et al. Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds. Burns Trauma 10:tkac001 (2022). PubMed: 35291229
    • Smith AJ  et al. GATD3A, a mitochondrial deglycase with evolutionary origins from gammaproteobacteria, restricts the formation of advanced glycation end products. BMC Biol 20:68 (2022). PubMed: 35307029
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MISS. Addie Luo

    Verified customer

    Submitted Nov 07 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AGE antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Antigen Retrival solution EDTA buffer pH9
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    No blocking step used for 15 minute(s) · Concentration: 0.03 · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 10 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-AGE antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (rat thoracic aorta)
    Specification
    rat thoracic aorta
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate buffer
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Hongfeng Wang

    Verified customer

    Submitted Aug 10 2010

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