Recombinant Anti-AICDA antibody [5G9] (ab252813)
![Western blot - Anti-AICDA antibody [5G9] (ab252813)](https://www.abcam.com/ps/products/252/ab252813/Images/ab252813-512652-anti-aicda-antibody-5g9-western-blot-wildtype-raji-aicda-knockout-raji-daudi-shsy5y.jpg "Western blot - Anti-AICDA antibody [5G9] (ab252813)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [5G9] to AICDA
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-AICDA antibody [5G9]
See all AICDA primary antibodies -
Description
Rat monoclonal [5G9] to AICDA -
Host species
Rat -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human AICDA. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: NAMALWA, Raji and WEHI-231 whole cell lysate. Flow Cyt (intra): Ramos cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5G9 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 0.316 µg/ml.
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| WB | (1) |
Use a concentration of 1.264 µg/ml. Predicted molecular weight: 23 kDa.
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| Notes |
|---|
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Flow Cyt (Intra)
Use a concentration of 0.316 µg/ml. |
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WB
Use a concentration of 1.264 µg/ml. Predicted molecular weight: 23 kDa. |
Target
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Function
RNA-editing deaminase involved in somatic hypermutation, gene conversion, and class-switch recombination. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. -
Tissue specificity
Strongly expressed in lymph nodes and tonsils. -
Involvement in disease
Defects in AICDA are the cause of hyper-IgM immunodeficiency syndrome type 2 (HIGM2) [MIM:605258]; also known as hyper-IgM syndrome 2. HIGM2 is an autosomal recessive disorder characterized by normal or elevated serum IgM levels with absence of IgG, IgA, and IgE, resulting in a profound susceptibility to bacterial infections. HIGM2 causes the absence of Ig class switch recombination (CSR), the lack of Ig somatic hypermutations, and lymph node hyperplasia caused by the presence of giant germinal centers. -
Sequence similarities
Belongs to the cytidine and deoxycytidylate deaminase family. - Information by UniProt
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Database links
- Entrez Gene: 57379 Human
- Entrez Gene: 11628 Mouse
- Omim: 605257 Human
- SwissProt: Q9GZX7 Human
- SwissProt: Q9WVE0 Mouse
- Unigene: 149342 Human
- Unigene: 391503 Mouse
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Alternative names
- Activation induced cytidine deaminase antibody
- Activation induced deaminase antibody
- Activation-induced cytidine deaminase antibody
see all
Images
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Western blot - Anti-AICDA antibody [5G9] (ab252813)All lanes : Anti-AICDA antibody [5G9] (ab252813) at 1.264 µg/ml
Lane 1 : Wild-type Raji cell lysate
Lane 2 : AICDA knockout Raji cell lysate
Lane 3 : Daudi cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-AICDA antibody [5G9] staining at 1.264 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab252813 was shown to bind specifically to AICDA. A band was observed at 22 kDa in wild-type Raji cell lysates with no signal observed at this size in AICDA knockout cell line ab277185 (knockout cell lysate ab277227). To generate this image, wild-type and AICDA knockout Raji cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
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![Western blot - Anti-AICDA antibody [5G9] (ab252813)](https://www.abcam.com/ps/products/252/ab252813/Images/ab252813-390662-anti-aicda-antibody-5g9-western-blot-namalwa.jpg)
Western blot - Anti-AICDA antibody [5G9] (ab252813)All lanes : Anti-AICDA antibody [5G9] (ab252813) at 1.264 µg/ml
Lane 1 : NAMALWA (human Burkitts lymphoma B lymphocyte), whole cell lysate
Lane 2 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate
Lane 4 : WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: K562 (PMID: 27217538).
Exposure time: 59 seconds.
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![Flow Cytometry (Intracellular) - Anti-AICDA antibody [5G9] (ab252813)](https://www.abcam.com/ps/products/252/ab252813/Images/ab252813-1-anti-aicda-antibody-5g9-flow-cytometry-k562.jpg)
Flow Cytometry (Intracellular) - Anti-AICDA antibody [5G9] (ab252813)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K-562 (human chronic myelogenous leukemia lymphoblast, Left) / Ramos (human Burkitt's lymphoma B lymphocyte, Right) cells labelling AICDA with ab252813 at0.316µg/mL (Red) compared with a rat monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rat IgG (Alexa Fluor® 488, ab150157) at 1/2000 dilution was used as the secondary antibody. Negative control: K-562.
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![Anti-AICDA antibody [5G9] (ab252813)](https://www.abcam.com/ps/products/252/ab252813/Images/ab252813-4-benefits-of-recombinant-antibodies.png)
Anti-AICDA antibody [5G9] (ab252813)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab252813 has been referenced in 1 publication.
- Greiner A et al. Differential expression of activation-induced cytidine deaminase (AID) in nodular lymphocyte-predominant and classical Hodgkin lymphoma. J Pathol 205:541-7 (2005). PubMed: 15732141