Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-AIF antibody [7F7AB10] (ab110327)

Reviews (1)Q&A (1)References (5)
Western blot - Anti-AIF antibody [7F7AB10] (ab110327)
  • Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] (ab110327)
  • Flow Cytometry - Anti-AIF antibody [7F7AB10] (ab110327)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] (ab110327)

Key features and details

  • Mouse monoclonal [7F7AB10] to AIF
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647

Overview

  • Product name

    Anti-AIF antibody [7F7AB10]
    See all AIF primary antibodies

  • Description

    Mouse monoclonal [7F7AB10] to AIF

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated mitochondria from Human heart; HeLa and HL60 cells. FFPE human heart tissue sections.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB (1)
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 67 kDa.
ICC/IF
Use a concentration of 5 - 10 µg/ml. For 2 hours.Paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes).
Notes
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB
Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 67 kDa.
ICC/IF
Use a concentration of 5 - 10 µg/ml. For 2 hours.Paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes).

Target

  • Function

    Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e., caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

  • Involvement in disease

    Defects in AIFM1 are the cause of combined oxidative phosphorylation deficiency type 6 (COXPD6) [MIM:300816]. It is a mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting.

  • Sequence similarities

    Belongs to the FAD-dependent oxidoreductase family.

  • Post-translational
    modifications

    Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.

  • Cellular localization

    Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm > perinuclear region. Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
  • Information by UniProt

  • Database links

    • Alternative names

      • AIFM1 antibody
      • AIFM1_HUMAN antibody
      • Apoptosis inducing factor 1, mitochondrial antibody
      • Apoptosis inducing factor antibody
      • Apoptosis inducing factor, mitochondrion associated, 1 antibody
      • Apoptosis-inducing factor 1 antibody
      • CMTX4 antibody
      • COWCK antibody
      • COXPD6 antibody
      • Harlequin antibody
      • Hq antibody
      • mAIF antibody
      • MGC111425 antibody
      • MGC5706 antibody
      • mitochondrial antibody
      • Neuropathy, axonal motor-sensory, with deafness and mental retardation antibody
      • neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome) antibody
      • PDCD 8 antibody
      • PDCD8 antibody
      • Programmed cell death 8 (apoptosis inducing factor) antibody
      • Programmed cell death 8 antibody
      • Programmed cell death 8 isoform 1 antibody
      • Programmed cell death 8 isoform 2 antibody
      • Programmed cell death 8 isoform 3 antibody
      • Programmed cell death protein 8 antibody
      • Programmed cell death protein 8 mitochondrial antibody
      • Programmed cell death protein 8 mitochondrial precursor antibody
      • Programmed cell death protein 8 mitochondrial precursor antibody
      • Striatal apoptosis inducing factor antibody
      see all

    Images

    • Western blot - Anti-AIF antibody [7F7AB10] (ab110327)
      Western blot - Anti-AIF antibody [7F7AB10] (ab110327)
      Anti-AIF antibody [7F7AB10] (ab110327) at 5 µg/ml + Isolated mitochondria from Human heart at 5 µg

      Predicted band size: 67 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] (ab110327)
      Immunocytochemistry/ Immunofluorescence - Anti-AIF antibody [7F7AB10] (ab110327)
      Immunocytochemistry analysis using ab110327 at 5µg/ml staining AIF in HeLa cells (4% paraformaldehyde fixed and Triton X-100 permeabilized). The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.
    • Flow Cytometry - Anti-AIF antibody [7F7AB10] (ab110327)
      Flow Cytometry - Anti-AIF antibody [7F7AB10] (ab110327)
      Flow cytometric analysis using ab110327 at 1µg/ml staining AIF in HL60 cells (blue). Isotype control antibody (red).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] (ab110327)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AIF antibody [7F7AB10] (ab110327)
      IHC image of AIF staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110327, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (5)

    Publishing research using ab110327? Please let us know so that we can cite the reference in this datasheet.

    ab110327 has been referenced in 5 publications.

    • Luo Q  et al. OTUD1 Activates Caspase-Independent and Caspase-Dependent Apoptosis by Promoting AIF Nuclear Translocation and MCL1 Degradation. Adv Sci (Weinh) 8:2002874 (2021). PubMed: 33898171
    • Cheng N  et al. Benzyl Isothiocyanate Induces Apoptosis and Inhibits Tumor Growth in Canine Mammary Carcinoma via Downregulation of the Cyclin B1/Cdk1 Pathway. Front Vet Sci 7:580530 (2020). PubMed: 33263014
    • Guida M  et al. Parkin Interacts with Apoptosis-Inducing Factor and Interferes with Its Translocation to the Nucleus in Neuronal Cells. Int J Mol Sci 20:N/A (2019). PubMed: 30754623
    • Wang X  et al. Subcellular NAMPT-mediated NAD+ salvage pathways and their roles in bioenergetics and neuronal protection after ischemic injury. J Neurochem 151:732-748 (2019). PubMed: 31553812
    • Luo T  et al. PARP-1 overexpression contributes to Cadmium-induced death in rat proximal tubular cells via parthanatos and the MAPK signalling pathway. Sci Rep 7:4331 (2017). PubMed: 28659599

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-AIF antibody [7F7AB10]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing (12.5)
    Sample
    Human Cell lysate - whole cell (Huh7)
    Specification
    Huh7
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 23 2013

    Question

    I tried the antibody and unfortunately it does not seem to recognise the cleaved or cytoplasmic form of AIF, which means the epitope must be within the first 103 amino acids of the protein.

    Read More
    Answer

    I would encourage you to submit an Abreview as this information will be useful for other customers, and you will receive Abpoints that can be redeemed against a future purchase.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com