Recombinant Anti-AK2 antibody [EPR11387(B)] (ab157206)
![Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)](https://www.abcam.com/ps/products/157/ab157206/Images/ab157206-395382-anti-ak2-antibody-epr11387-western-blot-human-knockout-lysate.jpg "Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11387(B)] to AK2
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AK2 antibody [EPR11387(B)]
See all AK2 primary antibodies -
Description
Rabbit monoclonal [EPR11387(B)] to AK2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, HepG2 and HL-60 cell lysates; Fetal kidney, heart and liver lysates; Recombinant human AK2 protein. IHC-P: Human heart and liver tissues. Flow Cyt (intra): HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11387(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis. -
Tissue specificity
Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level). -
Involvement in disease
Defects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1. -
Sequence similarities
Belongs to the adenylate kinase family. AK2 subfamily. -
Cellular localization
Mitochondrion intermembrane space. - Information by UniProt
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Database links
- Entrez Gene: 204 Human
- Omim: 103020 Human
- SwissProt: P54819 Human
- Unigene: 470907 Human
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Alternative names
- Adenylate kinase 2 antibody
- adenylate kinase 2, mitochondrial antibody
- Adenylate kinase isoenzyme 2 antibody
see all
Images
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Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : AK2 knockout HEK293T cell lysate
Lane 3 : HL-60 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 26 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab157206 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab157206 Anti-AK2 antibody [EPR11387(B)] was shown to specifically react with AK2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266539 (knockout cell lysate ab257825) was used. Wild-type and AK2 knockout samples were subjected to SDS-PAGE. ab157206 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)](https://www.abcam.com/ps/products/157/ab157206/Images/ab157206-164393-anti-ak2-antibody-epr11387b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue, labeling AK2 using ab157206 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-AK2 antibody [EPR11387(B)] (ab157206)](https://www.abcam.com/ps/products/157/ab157206/Images/ab157206-164391-anti-ak2-antibody-epr11387b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-AK2 antibody [EPR11387(B)] (ab157206)Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling AK2 (red), using ab157206 at a 1/10 dilution, and negative control cells probed with a Rabbit IgG (green)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)](https://www.abcam.com/ps/products/157/ab157206/Images/ab157206-164392-anti-ak2-antibody-epr11387b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11387(B)] (ab157206)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling AK2 using ab157206 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)](https://www.abcam.com/ps/products/157/ab157206/Images/ab157206-164394-anti-ak2-antibody-epr11387b-western-blot.jpg)
Western blot - Anti-AK2 antibody [EPR11387(B)] (ab157206)All lanes : Anti-AK2 antibody [EPR11387(B)] (ab157206) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : fetal kidney lysate
Lane 3 : fetal heart lysate
Lane 4 : HeLa lysate
Lane 5 : fetal liver lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Anti-AK2 antibody [EPR11387(B)] (ab157206)](https://www.abcam.com/ps/products/157/ab157206/Images/ab157206-6-benefits-of-recombinant-antibodies.png)
Anti-AK2 antibody [EPR11387(B)] (ab157206)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157206 has been referenced in 1 publication.
- Ghaloul-Gonzalez L et al. Reticular Dysgenesis and Mitochondriopathy Induced by Adenylate Kinase 2 Deficiency with Atypical Presentation. Sci Rep 9:15739 (2019). PubMed: 31673062