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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-AK2 antibody [EPR11388(B)] (ab166901)

Submit a review Submit a question References (2)
Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)
  • Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901)
  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)
  • Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)
  • Anti-AK2 antibody [EPR11388(B)] (ab166901)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11388(B)] to AK2
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-AK2 antibody [EPR11388(B)]
    See all AK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR11388(B)] to AK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human AK2. The exact sequence is proprietary.

  • Positive control

    • WB: Fetal kidney, Fetal liver, HEK-293T, HL-60, HepG2, MCF-7 and HeLa lysates. IHC-P: Paraffin-embedded Human kidney tissue and paraffin-embedded Human stomach tissue. ICC: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab166901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. This small ubiquitous enzyme involved in energy metabolism and nucleotide synthesis that is essential for maintenance and cell growth. Plays a key role in hematopoiesis.

  • Tissue specificity

    Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).

  • Involvement in disease

    Defects in AK2 are the cause of reticular dysgenesis (RDYS) [MIM:267500]; also known as aleukocytosis. RDYS is the most severe form of inborn severe combined immunodeficiencies (SCID) and is characterized by absence of granulocytes and almost complete deficiency of lymphocytes in peripheral blood, hypoplasia of the thymus and secondary lymphoid organs, and lack of innate and adaptive humoral and cellular immune functions, leading to fatal septicemia within days after birth. In bone marrow of individuals with reticular dysgenesis, myeloid differentiation is blocked at the promyelocytic stage, whereas erythro- and megakaryocytic maturation is generally normal.In addition, affected newborns have bilateral sensorineural deafness. Defects may be due to its absence in leukocytes and inner ear, in which its absence can not be compensated by AK1.

  • Sequence similarities

    Belongs to the adenylate kinase family. AK2 subfamily.

  • Cellular localization

    Mitochondrion intermembrane space.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Adenylate kinase 2 antibody
    • adenylate kinase 2, mitochondrial antibody
    • Adenylate kinase isoenzyme 2 antibody
    • ADK2 antibody
    • AK 2 antibody
    • ak2 antibody
    • ATP AMP transphosphorylase antibody
    • ATP-AMP transphosphorylase 2 antibody
    • EC 2.7.4.3 antibody
    • KAD2_HUMAN antibody
    • mitochondrial antibody
    see all

Images

  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    All lanes : Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : AK2 knockout HEK-293T cell lysate
    Lane 3 : HL-60 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab166901 observed at 26 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab166901 was shown to react with AK2 in wild-type HEK-293T cells in western blot with loss of signal observed in AK2 knockout cell line ab266539 (AK2 knockout cell lysate ab257825). Wild-type and AK2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab166901 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling AK2 using ab166901 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunocytochemistry/ Immunofluorescence - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunocytochemistry/Immunofluorescence analysis MCF-7 (human breast carcinoma) cells labelling AK2 with purified ab166901 at a dilution of 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) at dilution of 1/1000 was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Western blot - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    All lanes : Anti-AK2 antibody [EPR11388(B)] (ab166901) at 1/1000 dilution

    Lane 1 : Fetal kidney lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 26 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AK2 antibody [EPR11388(B)] (ab166901)

    Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling AK2 using ab166901 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunoprecipitation - Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Immunoprecipitation of AK2 from HeLa cell lysate pellet using ab166901 at 1/10 dilution followed by immunoblotting. HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-AK2 antibody [EPR11388(B)] (ab166901)
    Anti-AK2 antibody [EPR11388(B)] (ab166901)

References (2)

Publishing research using ab166901? Please let us know so that we can cite the reference in this datasheet.

ab166901 has been referenced in 2 publications.

  • Signes A  et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). PubMed: 30552096
  • Zhong Z  et al. New mitochondrial DNA synthesis enables NLRP3 inflammasome activation. Nature 560:198-203 (2018). PubMed: 30046112

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