Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)
![Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)](https://www.abcam.com/ps/products/131/ab131375/Images/ab131375-145806-anti-akr1c1-akr1c2-antibody-4b6af3-western-blot.jpg "Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)")
Key features and details
- Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-AKR1C1/AKR1C2 antibody [4B6AF3]
See all AKR1C1/AKR1C2 primary antibodies -
Description
Mouse monoclonal [4B6AF3] to AKR1C1/AKR1C2 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow
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Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- WB: HeLa, HepG2, HLH, HHH, MLH, RLH and RHH tissue samples ICC/IF: Hela cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituents: 0.877% Sodium chloride, 0.357% HEPES -
Concentration information loading... -
Purification notes
Purity >90% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4B6AF3 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131375 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa.
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| ICC/IF |
Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells)
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| Notes |
|---|
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 37 kDa. |
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ICC/IF
Use a concentration of 5 µg/ml. (paraformaldehyde fixed cells) |
Target
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Database links
- SwissProt: P52895 Human
- SwissProt: Q04828 Human
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Alternative names
- 20 alpha (3 alpha) hydroxysteroid dehydrogenase antibody
- 20-alpha-HSD antibody
- 20-alpha-hydroxysteroid dehydrogenase antibody
see all
Images
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Western blot - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)All lanes : Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375) at 2 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : HeLa Cell Lysate at 15 µg
Lane 3 : HepG2 Cell Lysate at 15 µg
Lane 4 : Human Liver Homogenate (HLH) at 10 µg
Lane 5 : Human Heart Homogenate (HHH) at 10 µg
Lane 6 : Mouse Liver Homogenate (MLH) at 10 µg
Lane 7 : Rat Liver Homogenate (RLH) at 10 µg
Lane 8 : Rat Heart Homogenate (RHH) at 10 µg
Secondary
All lanes : Goat anti-Mouse AP at 1/3000 dilution
Predicted band size: 37 kDa
0.3% PBST was used as a blocking buffer in all incubation steps. The high background signal in Mouse tissue sample was caused by the direct reaction between the Mouse IgG in Mouse tissue preps and the Goat anti-Mouse secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)](https://www.abcam.com/ps/products/131/ab131375/Images/ab131375-145805-anti-akr1c1-akr1c2-antibody-4b6af3-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKR1C1/AKR1C2 antibody [4B6AF3] (ab131375)Immunofluorescence analysis of AKR1C1 in HeLa cells, using ab131375 at 5 µg/ml.
The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 min). The cells were then incubated with ab131375 for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. The target protein locates to the cytosol.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab131375 has been referenced in 3 publications.
- Li C et al. High-Content Functional Screening of AEG-1 and AKR1C2 for the Promotion of Metastasis in Liver Cancer. J Biomol Screen 21:101-7 (2016). PubMed: 26318406
- Connarn JN et al. Metabolism of bupropion by carbonyl reductases in liver and intestine. Drug Metab Dispos 43:1019-27 (2015). PubMed: 25904761
- Li C et al. AEG-1 Promotes Metastasis Through Downstream AKR1C2 and NF1 in Liver Cancer. Oncol Res 22:203-211 (2014). PubMed: 26351209
