Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-AKT1 + AKT2 + AKT3 antibody
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT1 + AKT2 + AKT3 -
Host species
Rabbit -
Specificity
Recognizes AKT1, AKT2, and AKT3 proteins -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 aa 313-317.
Sequence:PEYLA
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Positive control
- 3T6 cell lysate and recombinant Human AKT1, AKT2, and AKT3
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 56 kDa.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 56 kDa. |
Target
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Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
Post-translational
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Omim: 164730 Human
- Omim: 164731 Human
see all -
Alternative names
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
Images
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)This image is courtesy of an anonymous AbreviewAll lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) at 1/5000 dilution
All lanes : Rat vascular smooth muscle cell lysate
Lysates/proteins at 100000 cells per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) at 1/1000 dilution
Lane 1 : Recombinant Human AKT1
Lane 2 : Recombinant Human AKT2
Lane 3 : Recombinant Human AKT3
Lysates/proteins at 0.015 µg per lane.
Predicted band size: 56 kDa -
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody (ab126811)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody (ab126811) at 1/1000 dilution
Lane 1 : 3T6 cell lysate at 3 µg
Lane 2 : 3T6 cell lysate at 10 µg
Lane 3 : 3T6 cell lysate at 30 µg
Predicted band size: 56 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab126811 has been referenced in 39 publications.
- Zhou J et al. The ILEI/LIFR complex induces EMT via the Akt and ERK pathways in renal interstitial fibrosis. J Transl Med 20:54 (2022). PubMed: 35093095
- Chiu YJ et al. Novel TRKB agonists activate TRKB and downstream ERK and AKT signaling to protect Aβ-GFP SH-SY5Y cells against Aβ toxicity. Aging (Albany NY) 14:7568-7586 (2022). PubMed: 36170028
- Jeon T et al. Silencing CDCA8 Suppresses Hepatocellular Carcinoma Growth and Stemness via Restoration of ATF3 Tumor Suppressor and Inactivation of AKT/ß-Catenin Signaling. Cancers (Basel) 13:N/A (2021). PubMed: 33801424
- Chen D et al. Suppressive effect of platycodin D on bladder cancer through microRNA-129-5p-mediated PABPC1/PI3K/AKT axis inactivation. Braz J Med Biol Res 54:e10222 (2021). PubMed: 33470388
- Alahverdi A et al. Involvement of EGFR, ERK-1,2 and AKT-1,2 Activity on Human Glioma Cell Growth. Asian Pac J Cancer Prev 21:3469-3475 (2020). PubMed: 33369441