Recombinant Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)
![Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-253716-anti-akt1-2-3-antibody-epr17737-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17737] to AKT1 + AKT2 + AKT3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
-
Product name
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737]
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit monoclonal [EPR17737] to AKT1 + AKT2 + AKT3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: AKT1, AKT2 and AKT3 recombinant protein fragments; MCF7, HepG2, PC-3, Jurkat, 293, C6 and PC-12 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, mouse spleen, rat brain and rat heart lysates. ICC/IF: MCF7 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17737 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
|
|
| ICC/IF |
1/1000.
|
| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
|
ICC/IF
1/1000. |
Target
-
Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
Post-translational
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
-
Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
see all -
Alternative names
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling AKT1 + AKT2 + AKT3 with ab200195 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Cytoplasm staining on MCF7 cell line is observed.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab200195 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-242534-anti-akt1-2-3-antibody-epr17737-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution
Lane 1 : AKT3 recombinant protein fragment (His-Tag®): aa351-479
Lane 2 : AKT2 recombinant protein fragment (His-Tag®): aa282-481
Lane 3 : AKT1 recombinant protein fragment (His-Tag®): aa281-480
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Exposure time: 3 secondsAKT3 recombinant protein fragment contains aa351-479 with His-Tag®. AKT2 recombinant protein fragment contains aa282-481 with His-Tag® and AKT1 recombinant protein fragment contains aa281-480 with His-Tag®.
Blocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-242533-anti-akt1-2-3-antibody-epr17737-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-242531-anti-akt1-2-3-antibody-epr17737-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/10000 dilution + HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-242530-anti-akt1-2-3-antibody-epr17737-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/10000 dilution
Lane 1 : PC-3 (Human prostate cancer cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : 293 (Human embryonic kidney) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-242529-anti-akt1-2-3-antibody-epr17737-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-242528-anti-akt1-2-3-antibody-epr17737-western-blot.jpg)
Western blot - Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)All lanes : Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)](https://www.abcam.com/ps/products/200/ab200195/Images/ab200195-10-benefits-of-recombinant-antibodies.png)
Anti-AKT1 + AKT2 + AKT3 antibody [EPR17737] (ab200195)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (9)
ab200195 has been referenced in 9 publications.
- Zhang L et al. Cyclovirobuxine D Induces Apoptosis and Mitochondrial Damage in Glioblastoma Cells Through ROS-Mediated Mitochondrial Translocation of Cofilin. Front Oncol 11:656184 (2021). PubMed: 33816313
- Qu Y et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182
- Li Z et al. The Immunostimulative Effect and Mechanisms of a Novel Mouse Anti-Human PD-1 Monoclonal Antibody on Jurkat Lymphocytic Cells Cocultured with Hepatoma Cells. Onco Targets Ther 13:12225-12241 (2020). PubMed: 33273828
- Wang C et al. Isoforsythiaside Attenuates Alzheimer's Disease via Regulating Mitochondrial Function Through the PI3K/AKT Pathway. Int J Mol Sci 21:N/A (2020). PubMed: 32784451
- Li L et al. Calf thymus polypeptide improved hematopoiesis via regulating colony-stimulating factors in BALB/c mice with hematopoietic dysfunction. Int J Biol Macromol 156:204-216 (2020). PubMed: 32156537
- Liu Y et al. Triterpenoids Extracted From Antrodia cinnamomea Mycelia Attenuate Acute Alcohol-Induced Liver Injury in C57BL/6 Mice via Suppression Inflammatory Response. Front Microbiol 11:1113 (2020). PubMed: 32719658
- Yang C et al. Anti-Diabetic Nephropathy Activities of Polysaccharides Obtained from Termitornyces albuminosus via Regulation of NF-?B Signaling in db/db Mice. Int J Mol Sci 20:N/A (2019). PubMed: 31640118
- Zheng D et al. FAK regulates epithelial-mesenchymal transition in adenomyosis. Mol Med Rep 18:5461-5472 (2018). PubMed: 30365102
- Liu C et al. Antidiabetic and Antinephritic Activities of Aqueous Extract of Cordyceps militaris Fruit Body in Diet-Streptozotocin-Induced Diabetic Sprague Dawley Rats. Oxid Med Cell Longev 2016:9685257 (2016). WB . PubMed: 27274781