Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody (ab183758)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human AKT1 + AKT2 + AKT3 aa 416-480 (phospho S472 + S473 + S474). The exact sequence is proprietary.
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Positive control
- MCF7 whole cell lysate/extract (500nM PMA treatment).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 56 kDa.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 56 kDa. |
Target
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Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
Post-translational
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Omim: 164730 Human
- Omim: 164731 Human
- Omim: 611223 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
see all -
Alternative names
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
Images
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Western blot - Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody (ab183758)All lanes : Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474) antibody (ab183758) at 1/500 dilution
Lane 1 : MCF7 whole cell lysate/extract (untreated)
Lane 2 : MCF7 whole cell lysate/extract (500nM PMA treatment for 1hr)
Lane 3 : MCF7 whole cell lysate/extract (500nM PMA treatment for 2hrs)
Lane 4 : MCF7 whole cell lysate/extract (500nM PMA treatment for 4hrs)
Lane 5 : MCF7 whole cell lysate/extract (500nM PMA treatment for 6hrs)
Lysates/proteins at 30 µg per lane.
Predicted band size: 56 kDa7.5 % SDS-PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab183758 has been referenced in 10 publications.
- Yamaguchi M et al. Prolactin Enhances the Proliferation of Proliferative Endometrial Glandular Cells and Endometrial Cancer Cells. J Endocr Soc 4:bvz029 (2020). PubMed: 32083234
- Yang Z et al. Long non-coding RNA HULC exerts oncogenic activity on papillary thyroid cancer in vitro and in vivo. Artif Cells Nanomed Biotechnol 48:326-335 (2020). PubMed: 31878795
- Wen Q et al. FoxO3a inhibiting expression of EPS8 to prevent progression of NSCLC: A new negative loop of EGFR signaling. EBioMedicine 40:198-209 (2019). PubMed: 30738830
- Wang X et al. Downregulation of GBAS regulates oral squamous cell carcinoma proliferation and apoptosis via the p53 signaling pathway. Onco Targets Ther 12:3729-3742 (2019). PubMed: 31190874
- Zhang J et al. Excessive miR-25-3p maturation via N6-methyladenosine stimulated by cigarette smoke promotes pancreatic cancer progression. Nat Commun 10:1858 (2019). PubMed: 31015415
- Li R et al. Heparin-Poloxamer Thermosensitive Hydrogel Loaded with bFGF and NGF Enhances Peripheral Nerve Regeneration in Diabetic Rats. Biomaterials 168:24-37 (2018). WB . PubMed: 29609091
- Ji Q et al. miR-216a inhibits osteosarcoma cell proliferation, invasion and metastasis by targeting CDK14. Cell Death Dis 8:e3103 (2017). PubMed: 29022909
- Lv L & Liu B Anti-tumor effects of bakuchiol on human gastric carcinoma cell lines are mediated through PI3K/AKT and MAPK signaling pathways. Mol Med Rep 16:8977-8982 (2017). PubMed: 28990045
- Wang Y et al. Stromal cell-derived factor-1a and transforming growth factor-ß1 synergistically facilitate migration and chondrogenesis of synovium-derived stem cells through MAPK pathways. Am J Transl Res 9:2656-2667 (2017). PubMed: 28560013
- Tayama S et al. The impact of EpCAM expression on response to chemotherapy and clinical outcomes in patients with epithelial ovarian cancer. Oncotarget 8:44312-44325 (2017). PubMed: 28574829