Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)
Key features and details
- Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody
See all AKT1 + AKT2 + AKT3 primary antibodies -
Description
Rabbit polyclonal to AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) -
Host species
Rabbit -
Specificity
ab131443 detects endogenous levels of AKT1/2/3 only when phosphorylated at tyrosine 315/316/312. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) conjugated to keyhole limpet haemocyanin.
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Positive control
- HepG2 cell extract treated with EGF; HeLa cells; Human breast carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131443 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 56 kDa.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF |
1/100 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 56 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/100 - 1/200. |
Target
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Function
IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent. -
Tissue specificity
In adult tissues, it is highly expressed in brain, lung and kidney, but weakly in heart, testis and liver. In fetal tissues, it is highly expressed in heart, liver and brain and not at all in kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. -
Post-translational
modificationsPhosphorylation on Thr-305 and Ser-472 is required for full activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. - Information by UniProt
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Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Omim: 164730 Human
- Omim: 164731 Human
- Omim: 611223 Human
- SwissProt: P31749 Human
- SwissProt: P31751 Human
see all -
Alternative names
- AKT antibody
- AKT1 antibody
- AKT1 kinase antibody
see all
Images
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Western blot - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)All lanes : Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443) at 1/500 dilution
Lane 1 : HepG2 cell extract untreated
Lane 2 : HepG2 cell extract treated with EGF
Predicted band size: 56 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)Immunofluorescent analysis of methanol-fixed HeLa cells labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 + AKT3 (phospho Y312 + Y315 + Y316) antibody (ab131443)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling AKT1 + AKT2 + AKT3 (phospho Y315 + Y316 + Y312) with ab131443 at 1/50 dilution. Right panel was preincubated with blocking peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (52)
ab131443 has been referenced in 52 publications.
- Shi YL et al. Trilobatin, a Natural Food Additive, Exerts Anti-Type 2 Diabetes Effect Mediated by Nrf2/ARE and IRS-1/GLUT2 Signaling Pathways. Front Pharmacol 13:828473 (2022). PubMed: 35153796
- Park KB et al. Leptin stimulates migration and invasion and maintains cancer stem‑like properties in gastric cancer cells. Oncol Rep 48:N/A (2022). PubMed: 35866593
- Sheng W et al. Curcumol inhibits the malignant progression of prostate cancer and regulates the PDK1/AKT/mTOR pathway by targeting miR‑9. Oncol Rep 46:N/A (2021). PubMed: 34590156
- Xia RM et al. RNA-binding protein RBM24 represses colorectal tumourigenesis by stabilising PTEN mRNA. Clin Transl Med 11:e383 (2021). PubMed: 34709758
- Peng T et al. Long non-coding RNA GAS5 suppresses rheumatoid arthritis progression via miR-128-3p/HDAC4 axis. Mol Cell Biochem 476:2491-2501 (2021). PubMed: 33611674