Recombinant Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)
![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264336-182729WB1.jpg "Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17062] to AKT1 + AKT2
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-AKT1 + AKT2 antibody [EPR17062]
See all AKT1 + AKT2 primary antibodies -
Description
Rabbit monoclonal [EPR17062] to AKT1 + AKT2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human AKT1 fragment recombinant protein; Human AKT2 fragment recombinant protein; Human fetal heart and fetal kidney lysates; HeLa, MCF7, C6, RAW 264.7 and NIH/3T3 whole cell lysates; Mouse and rat brain and heart lysates. IHC-P: Human tonsil, Human bladder cancer, mouse stomach and rat cerebrum tissues. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt (intra): HeLa and NIH/3T3 cells. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17062 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/600.
|
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
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| ICC/IF |
1/500.
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| IP |
1/40.
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| Notes |
|---|
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Flow Cyt (Intra)
1/600. |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
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ICC/IF
1/500. |
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IP
1/40. |
Target
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Relevance
The serine/threonine kinase AKT (protein kinase B or PKB) has a central role in the regulation of several signaling pathways controlling cell proliferation, apoptosis, angiogenesis, and diabetes. In humans, there are three genes in the "AKT family": AKT1, AKT2, and AKT3. AKT1 is catalytically inactive in serum starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet derived growth factor. The activation is rapid and specific. In the developing nervous system AKT is a critical mediator of growth factor induced neuronal survival. Survival factors can suppress apoptosis in a transcription independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. AKT2 is a putative oncogene and is a general protein kinase capable of phophorylating several known proteins. AKT2 is amplified and overexpressed in some human carcinomas. AKT2 acts primarily as a regulator of glucose metabolism. -
Cellular localization
ATK1: Cytoplasm. Nucleus. Cell membrane. Note: Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. -
Database links
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 11652 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 25233 Rat
- Omim: 164730 Human
- Omim: 164731 Human
see all -
Alternative names
- AKT 1 antibody
- AKT 2 antibody
- AKT antibody
see all
Images
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Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/1000 dilution
Lane 1 : Human AKT1 fragment recombinant protein
Lane 2 : Human AKT2 fragment recombinant protein
Lane 3 : Human AKT3 fragment recombinant protein
Lysates/proteins at 0.02 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human AKT1 fragment recombinant protein contains aa281-480 with a His-Tag®. Human AKT2 fragment recombinant protein contains aa282-481 with a His-Tag®. Human AKT3 fragment recombinant protein contains aa351-479 with a His-Tag®.
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![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264335-WB-2.jpg)
Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/5000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264334-WB-3.jpg)
Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 8 seconds; Lane 2: 2 seconds.
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![Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264333-182729WB4.jpg)
Western blot - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)All lanes : Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729) at 1/5000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat heart lysate
Lane 5 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1 2,3 and 4: 5 seconds; Lane 5,6 and 7: 3 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264332-182729IHCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Human tonsil is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264331-ihc-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Human bladder cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264330-ihc-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Mouse stomach is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264329-ihc-4.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling AKT1 + AKT2 with ab182729 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm and nuclear staining on Rat cerebrum is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264327-IF-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AKT1 + AKT2 with ab182729 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264326-IF-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling AKT1 + AKT2 with ab182729 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear and cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264325-FC-1.jpg)
Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling AKT1 + AKT2 with ab182729 at 1/600 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264324-FC-2.jpg)
Flow Cytometry (Intracellular) - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling AKT1 + AKT2 with ab182729 at 1/600 dilution (red) compared with a RabbitIgG,monoclonal [EPR25A]-Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-264323-182729ip1.jpg)
Immunoprecipitation - Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)AKT1/2 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab182729 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab182729 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10µg (Input).
Lane 2: ab182729 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab182729 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)](https://www.abcam.com/ps/products/182/ab182729/Images/ab182729-14-benefits-of-recombinant-antibodies.png)
Anti-AKT1 + AKT2 antibody [EPR17062] (ab182729)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (12)
ab182729 has been referenced in 12 publications.
- Wang S et al. miR-654-5p inhibits autophagy by targeting ATG7 via mTOR signaling in intervertebral disc degeneration. Mol Med Rep 23:N/A (2021). PubMed: 33846806
- Robbins Y et al. Dual PD-L1 and TGF-b blockade in patients with recurrent respiratory papillomatosis. J Immunother Cancer 9:N/A (2021). PubMed: 34462327
- Wu H et al. TIM-4 interference in Kupffer cells against CCL4-induced liver fibrosis by mediating Akt1/Mitophagy signalling pathway. Cell Prolif 53:e12731 (2020). PubMed: 31755616
- Li J & Zheng J Theaflavins prevent cartilage degeneration via AKT/FOXO3 signaling in vitro. Mol Med Rep 19:821-830 (2019). PubMed: 30569095
- Li F et al. CEP55 promotes cell proliferation and inhibits apoptosis via the PI3K/Akt/p21 signaling pathway in human glioma U251 cells. Oncol Lett 15:4789-4796 (2018). PubMed: 29552118