Anti-AKT1 antibody [9A4] (ab89402)
![Western blot - Anti-AKT1 antibody [9A4] (ab89402)](https://www.abcam.com/ps/products/89/ab89402/Images/ab89402-221913-anti-akt1-antibody-9a4-western-blot.jpg "Western blot - Anti-AKT1 antibody [9A4] (ab89402)")
Key features and details
- Mouse monoclonal [9A4] to AKT1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
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Overview
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Product name
Anti-AKT1 antibody [9A4]
See all AKT1 primary antibodies -
Description
Mouse monoclonal [9A4] to AKT1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length active protein
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Positive control
- HEK-293 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.5
Constituents: 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
9A4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab89402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/2500. Predicted molecular weight: 56 kDa.
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| Notes |
|---|
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WB
1/2500. Predicted molecular weight: 56 kDa. |
Target
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Function
Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. -
Tissue specificity
Expressed in all human cell types so far analyzed. The Tyr-176 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. -
Involvement in disease
Defects in AKT1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case.
Defects in AKT1 are associated with colorectal cancer (CRC) [MIM:114500].
Defects in AKT1 are associated with susceptibility to ovarian cancer [MIM:604370]; also called susceptibility to familial breast-ovarian cancer type 1 (BROVCA1). -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Domain
Binding of the PH domain to the phosphatidylinositol 3-kinase alpha (PI(3)K) results in its targeting to the plasma membrane. The PH domain mediates interaction with TNK2 and Tyr-176 is also essential for this interaction.
The AGC-kinase C-terminal mediates interaction with THEM4. -
Post-translational
modificationsPhosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Activated TNK2 phosphorylates it on Tyr-176 resulting in its binding to the anionic plasma membrane phospholipid PA. This phosphorylated form localizes to the cell membrane, where it is targeted by PDPK1 and PDPK2 for further phosphorylations on Thr-308 and Ser-473 leading to its activation. Ser-473 phosphorylation by mTORC2 favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with AGAP2 isoform 2 (PIKE-A). Ser-473 phosphorylation is enhanced in focal cortical dysplasias with Taylor-type balloon cells.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT1 ubiquitination is critical for phosphorylation and activation. When ubiquitinated, it translocates to the plasma membrane, where it becomes phosphorylated. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. -
Cellular localization
Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 207 Human
- Entrez Gene: 11651 Mouse
- Omim: 164730 Human
- SwissProt: P31749 Human
- SwissProt: P31750 Mouse
- Unigene: 525622 Human
- Unigene: 6645 Mouse
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Alternative names
- AKT 1 antibody
- AKT antibody
- AKT1 antibody
see all
Images
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Western blot - Anti-AKT1 antibody [9A4] (ab89402)
Primary : All Lanes : Anti AKT1 antibody (ab89402) at 1:2500 dilution. Lane 1 = AKT1 (His tag) full length recombinant protein ab62279 - 50ng. Lane 2 = NIH3T3 serum starved overnight 15ug. Lane 3 = NIH3T3 serum starved overnight and treated with PDGF-AB 50ng/mL for 1 hour 15ug. Secondary : Lanes 1-3 : Goat polyclonal to Mouse IgG H&L Pre-Adsorbed (HRP) at 1:5000 developed using the ECL technique. Performed under reducing conditions (50mM DTT Sample heated at 60°C). Predicted band size : 56kDa. Observed band size : 56kDa. Blocking step: 5% Milk in 50mM Tris+0.05% Tween for 1 hour at RT. Primary antibody buffer: 5% BSA in 50mM Tris+0.05% Tween overnight. Secondary antibody buffer: 5% Milk in 50mM Tris+0.05% Tween for 2 hours at RT. Exposure time : 5 minutes
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![Western blot - Anti-AKT1 antibody [9A4] (ab89402)](https://www.abcam.com/ps/products/89/ab89402/Images/ab89402-214679-anti-akt1-antibody-9a4-western-blot.jpg)
Western blot - Anti-AKT1 antibody [9A4] (ab89402)This image is courtesy of an anonymous AbreviewAll lanes : Anti-AKT1 antibody [9A4] (ab89402) at 1/2000 dilution
All lanes : HEK293 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti-mouse IgG polyclonal, undiluted
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
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![Western blot - Anti-AKT1 antibody [9A4] (ab89402)](https://www.abcam.com/ps/products/89/ab89402/Images/ab89402-105051-anti-akt1-antibody-9a4-western-blot.png)
Western blot - Anti-AKT1 antibody [9A4] (ab89402)All lanes : Anti-AKT1 antibody [9A4] (ab89402) at 1/2500 dilution
Lane 1 : HEK-293 cell lysate at 10 µg
Lane 2 : HEK-293 cell lysate at 20 µg
Lane 3 : HEK-293 cell lysate at 40 µg
Predicted band size: 56 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab89402 has been referenced in 7 publications.
- Ma Z et al. Repurposing a clinically approved prescription Colquhounia root tablet to treat diabetic kidney disease via suppressing PI3K/AKT/NF-kB activation. Chin Med 17:2 (2022). PubMed: 34980163
- Chen J et al. Network Pharmacology-Based Strategy for Elucidating the Molecular Basis Forthe Pharmacologic Effects of Licorice (Glycyrrhiza spp.). Front Pharmacol 12:590477 (2021). PubMed: 33995004
- Yin W et al. MG53 Inhibits the Progression of Tongue Cancer Cells through Regulating PI3K-AKT Signaling Pathway: Evidence from 3D Cell Culture and Animal Model. Small 15:e1805492 (2019). PubMed: 30690890
- Booth AD et al. Subcutaneous adipose tissue accumulation protects systemic glucose tolerance and muscle metabolism. Adipocyte 7:261-272 (2018). PubMed: 30230416
- Bastian C et al. CK2 inhibition confers functional protection to young and aging axons against ischemia by differentially regulating the CDK5 and AKT signaling pathways. Neurobiol Dis N/A:N/A (2018). PubMed: 29944965
- Lin Q et al. Cisplatin regulates cell autophagy in endometrial cancer cells via the PI3K/AKT/mTOR signalling pathway. Oncol Lett 13:3567-3571 (2017). PubMed: 28521459
- Huang Y et al. Phospho-?Np63a/miR-885-3p axis in tumor cell life and cell death upon cisplatin exposure. Cell Cycle 10:3938-47 (2011). PubMed: 22071691