Recombinant Anti-AKT2 antibody [EP1676] (ab131168)
![Western blot - Anti-AKT2 antibody [EP1676] (ab131168)](https://www.abcam.com/ps/products/131/ab131168/Images/ab131168-253295-anti-akt2-antibody-ep1676-western-blot.jpg "Western blot - Anti-AKT2 antibody [EP1676] (ab131168)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1676] to AKT2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-AKT2 antibody [EP1676]
See all AKT2 primary antibodies -
Description
Rabbit monoclonal [EP1676] to AKT2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat and 293T cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.20 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1676 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
General protein kinase capable of phosphorylating several known proteins. -
Tissue specificity
Expressed in all human cell types so far analyzed. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsPhosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
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Database links
- Entrez Gene: 208 Human
- Entrez Gene: 11652 Mouse
- Entrez Gene: 25233 Rat
- Omim: 164731 Human
- SwissProt: P31751 Human
- SwissProt: Q60823 Mouse
- SwissProt: P47197 Rat
- Unigene: 631535 Human
see all -
Alternative names
- AKT antibody
- Akt2 antibody
- AKT2_HUMAN antibody
see all
Images
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Western blot - Anti-AKT2 antibody [EP1676] (ab131168)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: AKT2 knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab131168 observed at 55 kDa. Red - loading control, ab8226, observed at 42 kDa.ab131168 was shown to recognize AKT2 when AKT2 knockout samples were used, along with additional cross-reactive bands. Wild-type and AKT2 knockout samples were subjected to SDS-PAGE. ab131168 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-AKT2 antibody [EP1676] (ab131168)](https://www.abcam.com/ps/products/131/ab131168/Images/ab131168-145447-anti-akt2-antibody-ep1676-western-blot.jpg)
Western blot - Anti-AKT2 antibody [EP1676] (ab131168)All lanes : Anti-AKT2 antibody [EP1676] (ab131168) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 55 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![OI-RD Scanning - Anti-AKT2 antibody [EP1676] (ab131168)](https://www.abcam.com/ps/products/131/ab131168/Images/ab131168-145446-anti-akt2-antibody-ep1676.jpg)
OI-RD Scanning - Anti-AKT2 antibody [EP1676] (ab131168) -
![Anti-AKT2 antibody [EP1676] (ab131168)](https://www.abcam.com/ps/products/131/ab131168/Images/ab131168-4-benefits-of-recombinant-antibodies.png)
Anti-AKT2 antibody [EP1676] (ab131168)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab131168 has been referenced in 2 publications.
- Xu X et al. Triptolide inhibits angiogenesis in microvascular endothelial cells through regulation of miR-92a. J Physiol Biochem 75:573-583 (2019). PubMed: 31691162
- Duan C et al. Sulfasalazine alters microglia phenotype by competing endogenous RNA effect of miR-136-5p and long non-coding RNA HOTAIR in cuprizone-induced demyelination. Biochem Pharmacol 155:110-123 (2018). PubMed: 29944870