Recombinant Anti-AKT3 + AKT1 antibody [E45] (ab32038)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.com/ps/products/32/ab32038/Images/ab32038-43706-anti-akt3-akt1-antibody-e45-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E45] to AKT3 + AKT1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-AKT3 + AKT1 antibody [E45] -
Description
Rabbit monoclonal [E45] to AKT3 + AKT1 -
Host species
Rabbit -
Specificity
ab32038 detects Akt1 and Akt3. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
-
Immunogen
Synthetic peptide corresponding to Human AKT1 aa 450-550.
Database link: P31749 -
Positive control
- Squamous lung carcinoma ICC/IF: SK-BR-3 cells
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E45 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| ICC/IF |
1/500.
|
| Notes |
|---|
|
IHC-P
1/250 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
Use at an assay dependent concentration. |
|
ICC/IF
1/500. |
Target
-
Cellular localization
AKT3: Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. AKT1: Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus. -
Database links
- Entrez Gene: 100137872 Cow
- Entrez Gene: 280991 Cow
- Entrez Gene: 10000 Human
- Entrez Gene: 207 Human
- Entrez Gene: 11651 Mouse
- Entrez Gene: 23797 Mouse
- Entrez Gene: 24185 Rat
- Entrez Gene: 29414 Rat
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT3 + AKT1 antibody [E45] (ab32038)
Immunohistochemistry (Paraffin-embedded sections) analysis using ab32038 at a dilution of 1/1000 - 1/1250 and squamous lung carcinoma
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.com/ps/products/32/ab32038/Images/ab32038-4-anti-akt3-akt1-antibody-e45-immunocytochemistry-skbr3-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AKT3 + AKT1 antibody [E45] (ab32038)Immunocytochemistry/ Immunofluorescence analysis of SK-BR-3 (human breast adenocarcinoma epithelial cell) cells labeling AKT3+AKT1 with purified ab32038 at 1/500 dilution (2.1 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
-
![Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.com/ps/products/32/ab32038/Images/ab32038-267109-anti-akt3-akt1-antibody-e45-western-blot.jpg)
Western blot - Anti-AKT3 + AKT1 antibody [E45] (ab32038)All lanes : Anti-AKT3 + AKT1 antibody [E45] (ab32038) at 1/10000 dilution
Lane 1 : GFP tagged AKT1 transfected 293T cell lysate
Lanes 2 & 4 & 6 : Empty vector transfected 293T cell lysate
Lane 3 : GFP tagged AKT2 transfected 293T cell lysate
Lane 5 : GFP tagged AKT3 transfected 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Exposure time: 1 secondBlocking/Diluting buffer: 5% NFDM/TBST.
Observed band: 82kDa, 56kDa.
82kDa band represents GFP tagged AKT. 56kDa band represents endogenous AKT.
-
![Anti-AKT3 + AKT1 antibody [E45] (ab32038)](https://www.abcam.com/ps/products/32/ab32038/Images/ab32038-5-benefits-of-recombinant-antibodies.png)
Anti-AKT3 + AKT1 antibody [E45] (ab32038)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (10)
ab32038 has been referenced in 10 publications.
- Li N et al. Cross-talk between TNF-a and IFN-? signaling in induction of B7-H1 expression in hepatocellular carcinoma cells. Cancer Immunol Immunother 67:271-283 (2018). PubMed: 29090321
- Yang CY et al. Melatonin exerts anti-oral cancer effect via suppressing LSD1 in patient-derived tumor xenograft models. Oncotarget 8:33756-33769 (2017). PubMed: 28422711
- Liao P et al. Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis. Elife 6:N/A (2017). WB . PubMed: 28440748
- Chen YC et al. High mobility group box 1-induced epithelial mesenchymal transition in human airway epithelial cells. Sci Rep 6:18815 (2016). WB ; Human . PubMed: 26739898
- Wang Y et al. Intrinsic cellular signaling mechanisms determine the sensitivity of cancer cells to virus-induced apoptosis. Sci Rep 6:37213 (2016). PubMed: 27849011
- Zhang Z et al. Upregulated periostin promotes angiogenesis in keloids through activation of the ERK 1/2 and focal adhesion kinase pathways, as well as the upregulated expression of VEGF and angiopoietin-1. Mol Med Rep 11:857-64 (2015). WB ; Human . PubMed: 25369801
- Verma R & Marchese A The endosomal sorting complex required for transport pathway mediates chemokine receptor CXCR4-promoted lysosomal degradation of the mammalian target of rapamycin antagonist DEPTOR. J Biol Chem 290:6810-24 (2015). PubMed: 25605718
- Miles L et al. Interaction between akt1-positive neurons and age at surgery is associated with surgical outcome in children with isolated focal cortical dysplasia. J Neuropathol Exp Neurol 72:884-91 (2013). PubMed: 23965747
- Feng M et al. Therapeutically targeting glypican-3 via a conformation-specific single-domain antibody in hepatocellular carcinoma. Proc Natl Acad Sci U S A 110:E1083-91 (2013). PubMed: 23471984
- Ulici V et al. The role of Akt1 in terminal stages of endochondral bone formation: angiogenesis and ossification. Bone 45:1133-45 (2009). WB ; Mouse . PubMed: 19679212