Anti-AL-1 antibody (ab70114)
Key features and details
- Rabbit polyclonal to AL-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AL-1 antibody
See all AL-1 primary antibodies -
Description
Rabbit polyclonal to AL-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human AL-1 (internal sequence).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab70114 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa).
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| IHC-P | (3) |
1/50 - 1/100.
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| ICC/IF | (1) |
1/500 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 26 kDa). |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500 - 1/1000. |
Target
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Function
May function actively to stimulate axon fasciculation. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. -
Sequence similarities
Belongs to the ephrin family. -
Cellular localization
Cell membrane. Membrane > caveola. Compartmentalized in discrete caveolae-like membrane microdomains. - Information by UniProt
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Database links
- Entrez Gene: 1946 Human
- Entrez Gene: 116683 Rat
- Omim: 601535 Human
- SwissProt: P52803 Human
- SwissProt: P97605 Rat
- Unigene: 288741 Human
- Unigene: 10714 Rat
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Alternative names
- EPLG7 antibody
- AF1 antibody
- AL 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AL-1 antibody (ab70114)This image is courtesy of an anonymous abreview.
ab70114 staining anti-AL-1 in wild-type and knockout mouse inferior colliculus/cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 15 minutes at 20°C; antigen retrieval was by heat mediation with citric acid. Samples were incubated with primary antibody (1/200 in 1 % TBS/BSA buffer) for 2 hours at 20°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-AL-1 antibody (ab70114)ab70114 at 1/500 dilution staining AL-1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AL-1 antibody (ab70114)ab70114 at 1/50 dilution staining AL-1 in human brain by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide
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Western blot - Anti-AL-1 antibody (ab70114)All lanes : Anti-AL-1 antibody (ab70114) at 1/500 dilution
Lane 1 : HeLa cell extracts
Lane 2 : HeLa cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab70114 has been referenced in 7 publications.
- Shen Q et al. Induction of innervation by encapsulated adipocytes with engineered vitamin A metabolism. Transl Res 192:1-14 (2018). WB . PubMed: 29144959
- Liu YH et al. Ascl1 promotes tangential migration and confines migratory routes by induction of Ephb2 in the telencephalon. Sci Rep 7:42895 (2017). PubMed: 28276447
- Son AI et al. Formation of persistent hyperplastic primary vitreous in ephrin-A5-/- mice. Invest Ophthalmol Vis Sci 55:1594-606 (2014). PubMed: 24550361
- Brennaman LH et al. Polysialylated NCAM and EphrinA/EphA Regulate Synaptic Development of GABAergic Interneurons in Prefrontal Cortex. Cereb Cortex : (2012). Mouse . PubMed: 22275477
- Kimura K et al. Pathway-specific engagement of ephrinA5-EphA4/EphA5 system of the substantia nigra pars reticulata in cocaine-induced responses. Proc Natl Acad Sci U S A 108:9981-6 (2011). IHC-Fr ; Mouse . PubMed: 21628570
- Stark DA et al. Eph/ephrin interactions modulate muscle satellite cell motility and patterning. Development 138:5279-89 (2011). Flow Cyt, IHC (PFA fixed) ; Mouse . PubMed: 22071104
- Afshari FT et al. Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J Neurosci 30:4246-55 (2010). WB, ICC/IF ; Rat . PubMed: 20335460