Recombinant Anti-Albumin antibody [EPR20195] (ab207327)
![Western blot - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-268830-anti-albumin-antibody-epr20195-western-blot.jpg "Western blot - Anti-Albumin antibody [EPR20195] (ab207327)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20195] to Albumin
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Albumin antibody [EPR20195]
See all Albumin primary antibodies -
Description
Rabbit monoclonal [EPR20195] to Albumin -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Human Albumin aa 1 to the C-terminus. (Purified Proteins from Normal Serum).
Database link: P02768 -
Positive control
- WB: Human, mouse and rat liver and plasma lysates. Human serum lysates. Human fetal kidney and spleen lysates. Mouse and rat spleen and kidney lysates. HepG2, NIH/3T3 and PC-12 whole cell lysates. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
Permeabilization is required. |
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| WB |
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
|
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| IP |
1/40.
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| ICC/IF |
1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. Permeabilization is required. |
|
WB
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa). |
|
IP
1/40. |
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ICC/IF
1/500. |
Target
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 213 Human
- Entrez Gene: 11657 Mouse
- Entrez Gene: 24186 Rat
- Omim: 103600 Human
- SwissProt: P02768 Human
- SwissProt: P07724 Mouse
- SwissProt: P02770 Rat
- Unigene: 418167 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
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Western blot - Anti-Albumin antibody [EPR20195] (ab207327)All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution
Lane 1 : Human liver lysate at 20 µg
Lane 2 : Mouse liver lysate at 20 µg
Lane 3 : Rat liver lysate at 20 µg
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-3: 5 seconds; Lane 4/5: 1 second.
Negative control: HeLa (PMID: 10476216 and 8314088).
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![Western blot - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-268829-anti-albumin-antibody-epr20195-western-blot.jpg)
Western blot - Anti-Albumin antibody [EPR20195] (ab207327)Lanes 1-4 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/10000 dilution
Lanes 5-6 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution
Lane 1 : Human plasma at 2 µg
Lane 2 : Rat plasma at 2 µg
Lane 3 : Mouse plasma at 2 µg
Lane 4 : Human serum at 2 µg
Lane 5 : Human fetal kidney lysate at 10 µg
Lane 6 : Human fetal spleen lysate at 10 µg
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 5-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-268828-anti-albumin-antibody-epr20195-western-blot.jpg)
Western blot - Anti-Albumin antibody [EPR20195] (ab207327)All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat spleen lysate
Lane 4 : Rat kidney lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 69 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-4: 1 second; Lane 5: 3 minutes; Lane 6: 5 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-268827-anti-albumin-antibody-epr20195-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HepG2 cell line.
Negative control: HeLa (PMID: 10476216 and 8314088)).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-268826-anti-albumin-antibody-epr20195-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (ab207327)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albuminwith ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-268825-anti-albumin-antibody-epr20195-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Albumin antibody [EPR20195] (ab207327)Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10µg (Input).
Lane 2: ab207327 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-340259-Albumin-3D-cleared-hepatocyte-culture.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)Albumin antibody ab207327 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D hepatocyte cell culture.
Blue: DAPI, Green: CD68, Yellow: Albumin, Red: Vimentin
Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section.
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![Anti-Albumin antibody [EPR20195] (ab207327)](https://www.abcam.com/ps/products/207/ab207327/Images/ab207327-8-benefits-of-recombinant-antibodies.png)
Anti-Albumin antibody [EPR20195] (ab207327)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (39)
ab207327 has been referenced in 39 publications.
- Su Q et al. Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aß clearance in APP/PS1 mice. Alzheimers Res Ther 13:7 (2021). PubMed: 33397436
- Dong J et al. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nat Commun 12:66 (2021). PubMed: 33397952
- Deng S et al. Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats. Oxid Med Cell Longev 2021:8891373 (2021). PubMed: 33708336
- Wang HQ et al. Folic acid alleviates jaundice of phenylhydrazine (PHA)-induced neonatal rats by reducing Lys-homocysteinylation of albumin. Cell Biol Toxicol N/A:N/A (2021). PubMed: 33788065
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873