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Cardiovascular Blood Serum Proteins
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RecombinantRabMAb

Recombinant Anti-Albumin antibody [EPR20195] (ab207327)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (39)

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Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
  • Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
  • Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
  • Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)
  • Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (ab207327)
  • Immunoprecipitation - Anti-Albumin antibody [EPR20195] (ab207327)
  • Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)
  • Anti-Albumin antibody [EPR20195] (ab207327)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20195] to Albumin
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Albumin antibody [EPR20195]
    See all Albumin primary antibodies
  • Description

    Rabbit monoclonal [EPR20195] to Albumin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Albumin aa 1 to the C-terminus. (Purified Proteins from Normal Serum).
    Database link: P02768

  • Positive control

    • WB: Human, mouse and rat liver and plasma lysates. Human serum lysates. Human fetal kidney and spleen lysates. Mouse and rat spleen and kidney lysates. HepG2, NIH/3T3 and PC-12 whole cell lysates. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20195
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
    • Anti-Albumin antibody [EPR20195] - BSA and Azide free (ab271979)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.

Permeabilization is required.

WB
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IP
1/40.
ICC/IF
1/500.
Notes
Flow Cyt (Intra)
1/500.

Permeabilization is required.

WB
1/2000. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).
IP
1/40.
ICC/IF
1/500.

Target

  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 213 Human
    • Entrez Gene: 11657 Mouse
    • Entrez Gene: 24186 Rat
    • Omim: 103600 Human
    • SwissProt: P02768 Human
    • SwissProt: P07724 Mouse
    • SwissProt: P02770 Rat
    • Unigene: 418167 Human
    • Unigene: 592379 Human
    • Unigene: 16773 Mouse
    • Unigene: 202968 Rat
    see all
  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • alb antibody
    • ALBU_HUMAN antibody
    • Albumin (32 AA) antibody
    • Albumin (AA 34) antibody
    • cell growth inhibiting protein 42 antibody
    • DKFZp779N1935 antibody
    • GIG20 antibody
    • GIG42 antibody
    • Growth inhibiting protein 20 antibody
    • growth-inhibiting protein 20 antibody
    • OTTHUMP00000220436 antibody
    • OTTHUMP00000220438 antibody
    • OTTHUMP00000220439 antibody
    • PRO0883 antibody
    • PRO0903 antibody
    • PRO1341 antibody
    • PRO1708 antibody
    • PRO2044 antibody
    • PRO2619 antibody
    • PRO2675 antibody
    • Serum albumin antibody
    • UNQ696/PRO1341 antibody
    see all

Images

  • Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
    Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
    All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

    Lane 1 : Human liver lysate at 20 µg
    Lane 2 : Mouse liver lysate at 20 µg
    Lane 3 : Rat liver lysate at 20 µg
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 5 seconds; Lane 4/5: 1 second.

    Negative control: HeLa (PMID: 10476216 and 8314088).

  • Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
    Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
    Lanes 1-4 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/10000 dilution
    Lanes 5-6 : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

    Lane 1 : Human plasma at 2 µg
    Lane 2 : Rat plasma at 2 µg
    Lane 3 : Mouse plasma at 2 µg
    Lane 4 : Human serum at 2 µg
    Lane 5 : Human fetal kidney lysate at 10 µg
    Lane 6 : Human fetal spleen lysate at 10 µg

    Secondary
    Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 5-6 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
    Western blot - Anti-Albumin antibody [EPR20195] (ab207327)
    All lanes : Anti-Albumin antibody [EPR20195] (ab207327) at 1/2000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat spleen lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 69 kDa
    Observed band size: 69 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 1 second; Lane 5: 3 minutes; Lane 6: 5 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)
    Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on HepG2 cell line.

    Negative control: HeLa (PMID: 10476216 and 8314088)).

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (ab207327)
    Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] (ab207327)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albuminwith ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-Albumin antibody [EPR20195] (ab207327)
    Immunoprecipitation - Anti-Albumin antibody [EPR20195] (ab207327)

    Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10µg (Input).

    Lane 2: ab207327 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

  • Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)
    Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] (ab207327)

    Albumin antibody ab207327 was used with 3D Cell Culture Clearing Kit ab243299 to penetrate, stain and clear a 3D hepatocyte cell culture.

    Blue: DAPI, Green: CD68, Yellow: Albumin, Red: Vimentin

    Learn more about 3D cell culture and tissue clearing kits, reagents, and protocols designed to make it easier to stain 3D cell cultures and thick tissue sections and get more data from each valuable tissue section.

  • Anti-Albumin antibody [EPR20195] (ab207327)
    Anti-Albumin antibody [EPR20195] (ab207327)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (39)

Publishing research using ab207327? Please let us know so that we can cite the reference in this datasheet.

ab207327 has been referenced in 39 publications.

  • Su Q  et al. Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aß clearance in APP/PS1 mice. Alzheimers Res Ther 13:7 (2021). PubMed: 33397436
  • Dong J  et al. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nat Commun 12:66 (2021). PubMed: 33397952
  • Deng S  et al. Albumin Reduces Oxidative Stress and Neuronal Apoptosis via the ERK/Nrf2/HO-1 Pathway after Intracerebral Hemorrhage in Rats. Oxid Med Cell Longev 2021:8891373 (2021). PubMed: 33708336
  • Wang HQ  et al. Folic acid alleviates jaundice of phenylhydrazine (PHA)-induced neonatal rats by reducing Lys-homocysteinylation of albumin. Cell Biol Toxicol N/A:N/A (2021). PubMed: 33788065
  • Zhang X  et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

IHC - Wholemount abreview for Anti-Albumin antibody [EPR20195]

Inconclusive
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Tissue (brain)
Specification
brain
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 28 2019

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