Recombinant Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
![Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)](https://www.abcam.com/ps/products/222/ab222923/Images/ab222923-321023-anti-albumin-antibody-epr20195-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20195] to Albumin - Low endotoxin, Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free
See all Albumin primary antibodies -
Description
Rabbit monoclonal [EPR20195] to Albumin - Low endotoxin, Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Full length native protein (purified) corresponding to Human Albumin aa 1 to the C-terminus. (Purified Proteins from Normal Serum).
Database link: P02768 -
Positive control
- WB: Human, mouse and rat liver and plasma lysates. Human serum lysates. Human fetal kidney and spleen lysates. Mouse and rat spleen and kidney lysates. HepG2, NIH/3T3 and PC-12 whole cell lysates. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
ab222923 is the carrier-free version of ab207327.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20195 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
Permeabilization is required. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. Permeabilization is required. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
Target
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Function
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
Sequence similarities
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 213 Human
- Entrez Gene: 11657 Mouse
- Entrez Gene: 24186 Rat
- Omim: 103600 Human
- SwissProt: P02768 Human
- SwissProt: P07724 Mouse
- SwissProt: P02770 Rat
- Unigene: 418167 Human
see all -
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- alb antibody
- ALBU_HUMAN antibody
- Albumin (32 AA) antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) or HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Albumin with ab207327 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing cytoplasmic staining on HepG2 cell line.
Negative control: HeLa (PMID: 10476216 and 8314088)).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).
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![Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)](https://www.abcam.com/ps/products/222/ab222923/Images/ab222923-321022-anti-albumin-antibody-epr20195-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Albumin with ab207327 at 1/500 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).
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![Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)](https://www.abcam.com/ps/products/222/ab222923/Images/ab222923-321021-anti-albumin-antibody-epr20195-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)Albumin was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207327 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207327 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HepG2 whole cell lysate, 10µg (Input).
Lane 2: ab207327 IP in HepG2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207327 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207327).
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![Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)](https://www.abcam.com/ps/products/222/ab222923/Images/ab222923-4-benefits-of-recombinant-antibodies.png)
Anti-Albumin antibody [EPR20195] - Low endotoxin, Azide free (ab222923)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab222923 has been referenced in 1 publication.
- Kim B et al. SYNCRIP controls miR-137 and striatal learning in animal models of methamphetamine abstinence. Acta Pharm Sin B 12:3281-3297 (2022). PubMed: 35967275
