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RecombinantRabMAb

Recombinant Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)

Reviews (1) Submit a question References (1)
Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
  • Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4440] to Alcohol Dehydrogenase
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Alcohol Dehydrogenase antibody [EPR4440]
    See all Alcohol Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EPR4440] to Alcohol Dehydrogenase

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, rat liver and mouse liver lysates IHC-P: Human liver tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Heat up to 98 °C, below boiling, and then let cool for 10-20 minutes.
Application notes
Is unsuitable for Flow Cyt or ICC/IF.

Target

Images

  • Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
    Western blot - Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
    All lanes : Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)

    Immunohistochemical staining of Alcohol Dehydrogenase in paraffin embedded Human liver tissue, using ab108197 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)
    Anti-Alcohol Dehydrogenase antibody [EPR4440] (ab108197)

References (1)

Publishing research using ab108197? Please let us know so that we can cite the reference in this datasheet.

ab108197 has been referenced in 1 publication.

  • Mouton AJ  et al. Alcohol Vapor Inhalation as a Model of Alcohol-Induced Organ Disease. Alcohol Clin Exp Res 40:1671-8 (2016). PubMed: 27375174

Customer reviews and Q&As

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Western blot abreview for Anti-Alcohol Dehydrogenase antibody [EPR4440]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (white adipose tissue)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
30 µg
Specification
white adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. William Zhang

Verified customer

Submitted Mar 06 2018

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