Recombinant Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
![Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)](https://www.abcam.com/ps/products/250/ab250728/Images/ab250728-450548-anti-aldehyde-dehydrogenase-10-antibody-epr15425b-bsa-and-azide-free-western-blot-wildetype-HeLa-aldh3a2-knockout-HeLa.jpg "Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free
See all Aldehyde dehydrogenase 10 primary antibodies -
Description
Rabbit monoclonal [EPR15425(B)] to Aldehyde dehydrogenase 10 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A549, 293T and U87-MG cell lysates; A549 and HepG2 cells.
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General notes
ab250728 is the carrier-free version of ab184171.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15425(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| WB |
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
Target
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Function
Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length. -
Involvement in disease
Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS) [MIM:270200]. SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization). Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 224 Human
- Entrez Gene: 11671 Mouse
- Omim: 609523 Human
- SwissProt: P51648 Human
- SwissProt: P47740 Mouse
- Unigene: 499886 Human
- Unigene: 398221 Mouse
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Alternative names
- Ahd 3 antibody
- Ahd 3r antibody
- Ahd3 antibody
see all
Images
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Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ALDH3A2 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab184171).
Lanes 1 - 2: Merged signal (red and green). Green - ab184171 observed at 50 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab184171 was shown to react with ALDH3A2 in wild-type HeLa cells in Western blot with loss of signal observed in ALDH3A2 knockout cell line ab265427 (ALDH3A2 knockout cell lysate ab257829). Wild-type and ALDH3A2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab184171 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)](https://www.abcam.com/ps/products/250/ab250728/Images/ab250728-3-anti-aldehyde-dehydrogenase-10-antibody-epr15425-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)This data was developed using ab184171, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of 4% paraformaldehyde fixed A549 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/250 dilution (red). A Goat anti rabbit IgG (Alexa Fluor555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue). -
![Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)](https://www.abcam.com/ps/products/250/ab250728/Images/ab250728-2-anti-aldehyde-dehydrogenase-10-antibody-epr15425-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)This data was developed using ab184171, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HepG2 cells labeling Aldehyde dehydrogenase 10 using ab184171 at 1/170 dilution (red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Isotype control: Rabbit monoclonal IgG (green).
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![Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)](https://www.abcam.com/ps/products/250/ab250728/Images/ab250728-398193-anti-aldehyde-dehydrogenase-10-antibody-epr15425-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/10000 dilution + U87-MG cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDaThis data was developed using ab184171, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)](https://www.abcam.com/ps/products/250/ab250728/Images/ab250728-407982-anti-aldehyde-dehydrogenase-10-antibody-epr15425-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)All lanes : Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] (ab184171) at 1/2000 dilution
Lane 1 : A549 cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDaThis data was developed using ab184171, the same antibody clone in a different buffer formulation.
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![Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)](https://www.abcam.com/ps/products/250/ab250728/Images/ab250728-5-benefits-of-recombinant-antibodies.png)
Anti-Aldehyde dehydrogenase 10 antibody [EPR15425(B)] - BSA and Azide free (ab250728)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250728 has not yet been referenced specifically in any publications.
