Anti-ALDH1A3 antibody (ab129815)
Key features and details
- Rabbit polyclonal to ALDH1A3
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-ALDH1A3 antibody
See all ALDH1A3 primary antibodies -
Description
Rabbit polyclonal to ALDH1A3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, African green monkey, Chinese hamster, Syrian hamster, Orangutan
-
Immunogen
Synthetic peptide corresponding to Human ALDH1A3 aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab154771) -
Positive control
- This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates. IHC-P: human stomach tissue sections. ICC/IF: PC3
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (7) |
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
|
| IP |
Use a concentration of 5 µg/ml.
|
|
| ICC/IF |
Use a concentration of 1 - 5 µg/ml.
|
|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
|
IP
Use a concentration of 5 µg/ml. |
|
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
|
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 220 Human
- Entrez Gene: 56847 Mouse
- Entrez Gene: 266603 Rat
- Omim: 600463 Human
- SwissProt: P47895 Human
- SwissProt: Q9JHW9 Mouse
- SwissProt: Q8K4D8 Rat
- Unigene: 459538 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 6 antibody
- AL1A3_HUMAN antibody
- Aldehyde dehydrogenase 1A3 antibody
see all
Images
-
Western blot - Anti-ALDH1A3 antibody (ab129815)All lanes : Anti-ALDH1A3 antibody (ab129815) at 1 µg/ml
Lane 1 : Human kidney tissue lysate - total protein (ab30203)
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 12 kDa, 32 kDa, 37 kDa, 47 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129815 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1A3 antibody (ab129815)IHC image of ab129815 staining in human stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab129815, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-ALDH1A3 antibody (ab129815)ab129815 staining ALDH1A3 in PC3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab129815 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
Immunoprecipitation - Anti-ALDH1A3 antibody (ab129815)ALDH1A3 was immunoprecipitated using 0.5mg Mouse Kidney tissue lysate, 5µg of Rabbit polyclonal to ALDH1A3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab129815.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 56kDa; ALDH1A3
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (24)
ab129815 has been referenced in 24 publications.
- Chen Q et al. ADP-Ribosylation Factor Like GTPase 4C (ARL4C) augments stem-like traits of glioblastoma cells by upregulating ALDH1A3. J Cancer 12:818-826 (2021). PubMed: 33403039
- Lee JS et al. Overall survival of pancreatic ductal adenocarcinoma is doubled by Aldh7a1 deletion in the KPC mouse. Theranostics 11:3472-3488 (2021). PubMed: 33537098
- Liu G et al. Long non-coding RNA DPP10-AS1 exerts anti-tumor effects on colon cancer via the upregulation of ADCY1 by regulating microRNA-127-3p. Aging (Albany NY) 13:9748-9765 (2021). PubMed: 33744851
- Kafarnik C et al. Canine Corneal Stromal Cells Have Multipotent Mesenchymal Stromal Cell Properties In Vitro. Stem Cells Dev 29:425-439 (2020). PubMed: 31973649
- Tian T et al. BET degrader inhibits tumor progression and stem-like cell growth via Wnt/ß-catenin signaling repression in glioma cells. Cell Death Dis 11:900 (2020). PubMed: 33093476