Recombinant Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-205729-anti-aldh1l1-antibody-epr12743b-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12743(B)] to ALDH1L1 - Astrocyte Marker
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker
See all ALDH1L1 primary antibodies -
Description
Rabbit monoclonal [EPR12743(B)] to ALDH1L1 - Astrocyte Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ALDH1L1 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: O75891 -
Positive control
- HepG2, 293T, fetal brain, fetal kidney and fetal liver lysates. Human kidney and Human liver tissue. IHC-Fr: Mouse and rat liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12743(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
|
|
| IHC-Fr |
1/100.
|
| Notes |
|---|
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. |
|
IHC-Fr
1/100. |
Application notes
Is unsuitable for Flow Cyt or ICC/IF.
Target
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Tissue specificity
Highly expressed in liver, pancreas and kidney. -
Sequence similarities
In the N-terminal section; belongs to the GART family.
In the C-terminal section; belongs to the aldehyde dehydrogenase family. ALDH1L subfamily.
Contains 1 acyl carrier domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10840 Human
- Entrez Gene: 107747 Mouse
- Entrez Gene: 64392 Rat
- Omim: 600249 Human
- SwissProt: O75891 Human
- SwissProt: Q8R0Y6 Mouse
- SwissProt: P28037 Rat
- Unigene: 434435 Human
see all -
Alternative names
- 10 formyltetrahydrofolate dehydrogenase antibody
- 10 FTHFDH antibody
- 10-formyltetrahydrofolate dehydrogenase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-205728-anti-aldh1l1-antibody-epr12743b-western-blot.jpg)
Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)All lanes : Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T cell lysate
Lane 3 : Fetal brain lysate
Lane 4 : Fetal kidney lysate
Lane 5 : Fetal liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 99 kDa -
![Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-394070-anti-aldh-1l1-antibody-epr12743-b-immunohistochemistry-liver-mouse.jpg)
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)Immunohistochemistry (Frozen sections) analysis of mouse liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).Cytoplasmic staining on mouse liver. -
![Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-394069-anti-aldh-1l1-antibody-epr12743-b-immunohistochemistry-liver-rat.jpg)
Immunohistochemistry (Frozen sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)Immunohistochemistry (Frozen sections) analysis of rat liver tissue labeling ALDH1L1 with ab177463 at 1/100 dilution. Tissue was fixed with 4% PFA and permeabilised with 0.2% Triton X-100. ab150077, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. DAPI (blue) was used as nucleare counterstain. Antigen retrieval was heat mediated using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).Cytoplasmic staining on rat liver. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-205730-anti-aldh1l1-antibody-epr12743b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ALDH1L1 with ab177463 at 1/50 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-205731-anti-aldh1l1-antibody-epr12743b-western-blot.jpg)
Western blot - Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)All lanes : Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463) at 1/1000 dilution
Lane 1 : Fetal liver lysate at 10 µg
Lane 2 : 1X PBS (negative control)
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 99 kDa -
![Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)](https://www.abcam.com/ps/products/177/ab177463/Images/ab177463-7-benefits-of-recombinant-antibodies.png)
Anti-ALDH1L1 antibody [EPR12743(B)] - Astrocyte Marker (ab177463)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab177463 has been referenced in 6 publications.
- Zhong Y & Lan J Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78. Bioengineered 13:1952-1961 (2022). Human . PubMed: 35104170
- Dobolyi A et al. Exclusive neuronal detection of KGDHC-specific subunits in the adult human brain cortex despite pancellular protein lysine succinylation. Brain Struct Funct 225:639-667 (2020). PubMed: 31982949
- Hanshaw DM et al. Large felid leucoencephalomyelopathy in a Sumatran tiger (Panthera tigris sumatrae) from an Australian zoo. Aust Vet J 97:277-282 (2019). PubMed: 31209862
- Milinkeviciute G et al. Microglia Regulate Pruning of Specialized Synapses in the Auditory Brainstem. Front Neural Circuits 13:55 (2019). PubMed: 31555101
- Peter CJ et al. In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene. Nat Commun 10:4112 (2019). PubMed: 31511512
- Raddatz BB et al. Transcriptional analysis of glial cell differentiation in the postnatal murine spinal cord. Int J Dev Neurosci 42:24-36 (2015). IHC-P ; Mouse . PubMed: 25702526