Recombinant Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)
![Western blot - Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)](https://www.abcam.com/ps/products/248/ab248267/Images/ab248267-402344-anti-aldh3a1-antibody-epr7406-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7406] to ALDH3A1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free
See all ALDH3A1 primary antibodies -
Description
Rabbit monoclonal [EPR7406] to ALDH3A1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248267 is the carrier-free version of ab129022.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 9.00 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7406 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. -
Tissue specificity
High levels in stomach, esophagus and lung; low level in the liver and kidney. -
Sequence similarities
Belongs to the aldehyde dehydrogenase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 218 Human
- Entrez Gene: 11670 Mouse
- Entrez Gene: 25375 Rat
- Omim: 100660 Human
- SwissProt: P30838 Human
- SwissProt: P47739 Mouse
- SwissProt: P11883 Rat
- Unigene: 531682 Human
see all -
Alternative names
- Acetaldehyde dehydrogenase 3 antibody
- AHD 4 antibody
- AHD C antibody
see all
Images
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Western blot - Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)Anti-ALDH3A1 antibody [EPR7406] (ab129022) at 1/1000 dilution + Rat eyeball lysates at 10 µg
Secondary
HRP-conjugated goat anti-abbit at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDaThis data was developed using ab129022, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)](https://www.abcam.com/ps/products/248/ab248267/Images/ab248267-1-anti-aldh3a1-antibody-epr7406-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)This data was developed using ab129022, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)](https://www.abcam.com/ps/products/248/ab248267/Images/ab248267-3-benefits-of-recombinant-antibodies.png)
Anti-ALDH3A1 antibody [EPR7406] - BSA and Azide free (ab248267)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248267 has not yet been referenced specifically in any publications.
