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Recombinant

Recombinant Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
  • Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP298] to ALDH3A1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

Overview

  • Product name

    Anti-ALDH3A1 antibody [SP298] - BSA and Azide free
    See all ALDH3A1 primary antibodies

  • Description

    Rabbit monoclonal [SP298] to ALDH3A1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549 cell lysate. IHC-P: Human bladder, esophagus, skin, cervix, tonsil, stomach, stomach adenocarcinoma, lung squamous cell carcinoma and cervical squamous cell carcinoma tissues.

  • General notes

    ab272015 is the carrier-free version of ab227694.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
Notes
IHC-P
Use at an assay dependent concentration.

Boil tissue section in citrate buffer pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Incubate with primary antibody for 10 minutes at room temperature.

 
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.

Target

  • Function

    ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes.

  • Tissue specificity

    High levels in stomach, esophagus and lung; low level in the liver and kidney.

  • Sequence similarities

    Belongs to the aldehyde dehydrogenase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Acetaldehyde dehydrogenase 3 antibody
      • AHD 4 antibody
      • AHD C antibody
      • AHD4 antibody
      • AHDC antibody
      • AL3A1_HUMAN antibody
      • Aldehyde dehydrogenase 3 antibody
      • Aldehyde dehydrogenase 3 family member A1 antibody
      • Aldehyde dehydrogenase 3A1 antibody
      • Aldehyde dehydrogenase antibody
      • Aldehyde dehydrogenase class 3 antibody
      • Aldehyde dehydrogenase dimeric NADP preferring antibody
      • Aldehyde dehydrogenase family 3 member A1 antibody
      • Aldehyde dehydrogenase family 3 subfamily A1 antibody
      • Aldehyde dehydrogenase family 3, subfamily A, member 1 antibody
      • Aldehyde dehydrogenase isozyme 3 antibody
      • Aldehyde dehydrogenase type III antibody
      • ALDH 3 antibody
      • ALDH 3A1 antibody
      • ALDH III antibody
      • ALDH, stomach type antibody
      • ALDH3 A1 antibody
      • ALDH3 antibody
      • ALDH3A 1 antibody
      • Aldh3a1 antibody
      • ALDHIII antibody
      • dimeric NADP-preferring antibody
      • MGC10406 antibody
      • Stomach aldehyde dehydrogenase antibody
      • Tumor associated aldehyde dehydrogenase tumor ALDH antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human cervix tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human skin tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human esophagus tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human bladder tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human stomach tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for ALDH3A1 using ab227694 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227694).
    • Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)
      Anti-ALDH3A1 antibody [SP298] - BSA and Azide free (ab272015)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab272015? Please let us know so that we can cite the reference in this datasheet.

    ab272015 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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