Recombinant Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
![Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)](https://www.abcam.com/ps/products/75/ab75751/Images/ab75751-438736-AntiAldolase-B-antibody.jpg "Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Aldolase B + Aldolase C antibody [EPR3138Y]
See all Aldolase B+Aldolase C primary antibodies -
Description
Rabbit monoclonal [EPR3138Y] to Aldolase B + Aldolase C -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Aldolase B aa 250-350. The exact sequence is proprietary.
Database link: P05062 -
Positive control
- IHC: Human, mouse and rat kidney tissues. WB: Human, mouse and rat kidney lysate. His-tagged human Aldolase B and C full-length recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3138Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000. Predicted molecular weight: 39 kDa.
For unpurified use at 1/500 - 1/1000. |
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000. Predicted molecular weight: 39 kDa. For unpurified use at 1/500 - 1/1000. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Images
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Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/200 dilution
Lane 1 : His-tagged human Aldolase A full-length recombinant protein
Lane 2 : His-tagged human Aldolase B full-length recombinant protein
Lane 3 : His-tagged human Aldolase C full-length recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST
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![Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)](https://www.abcam.com/ps/products/75/ab75751/Images/ab75751-420468-aldolase-b-antibody-epr3138y-westernblot-human-mouse-rat.jpg)
Western blot - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)All lanes : Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751) at 1/1000 dilution (Purified)
Lane 1 : Human kidney lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDaBlocking Buffer and concentration: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)](https://www.abcam.com/ps/products/75/ab75751/Images/ab75751-9-aldolase-b-antibody-epr3138y-immunohistochemistry-kidney-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)](https://www.abcam.com/ps/products/75/ab75751/Images/ab75751-8-aldolase-b-antibody-epr3138y-immunohistochemistry-kidney-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)](https://www.abcam.com/ps/products/75/ab75751/Images/ab75751-7-aldolase-b-antibody-epr3138y-immunohistochemistry-kidney-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Aldolase B with Purified ab75751 at 1:100 dilution (1.47 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)](https://www.abcam.com/ps/products/75/ab75751/Images/ab75751-10-benefits-of-recombinant-antibodies.png)
Anti-Aldolase B + Aldolase C antibody [EPR3138Y] (ab75751)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab75751 has been referenced in 2 publications.
- Wang C et al. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLoS One 9:e101315 (2014). WB . PubMed: 24983750
- Tun HW et al. Pathway signature and cellular differentiation in clear cell renal cell carcinoma. PLoS One 5:e10696 (2010). IHC-P ; Human . PubMed: 20502531