Anti-Aldolase B antibody [5E2AD2] (ab129728)
![Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-4.jpg "Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)")
Key features and details
- Mouse monoclonal [5E2AD2] to Aldolase B
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Aldolase B antibody [5E2AD2]
See all Aldolase B primary antibodies -
Description
Mouse monoclonal [5E2AD2] to Aldolase B -
Host species
Mouse -
Tested applications
Suitable for: IP, ICC/IF, Flow Cytmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Human, Rat and Mouse liver homogenate lysates; HepG2 cells; Hela cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab129728 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
5E2AD2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| Flow Cyt |
Use a concentration of 5 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use a concentration of 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Pathway
Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. -
Involvement in disease
Defects in ALDOB are the cause of hereditary fructose intolerance (HFI) [MIM:229600]. HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. -
Sequence similarities
Belongs to the class I fructose-bisphosphate aldolase family. - Information by UniProt
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Database links
- Entrez Gene: 229 Human
- Entrez Gene: 230163 Mouse
- Entrez Gene: 24190 Rat
- Omim: 612724 Human
- SwissProt: P05062 Human
- SwissProt: Q91Y97 Mouse
- SwissProt: P00884 Rat
- Unigene: 530274 Human
see all -
Alternative names
- ALDB antibody
- ALDO B antibody
- ALDO2 antibody
see all
Images
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Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)Immunoprecipitation image of ab129728. In vertebrates, three forms of this ubiquitous glycolytic enzyme are found, aldolase A in muscle, aldolase B in liver and aldolase C in brain. Antibody ab129728 immunocaptured 39.4 kDa ALDOB only from rat liver homogenate (RLH), not from rat brain homogenate (RBH) nor rat skeletal muscle homogenate (RSMH). Gel stained with Coomassie brilliant blue G. Lane1: ladder Lane2: rat brain homogenate lysate Lane3: rat liver homogenate lysate Lane4: rat skeletal muscle homogenate lysate
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![Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-1.jpg)
Immunoprecipitation - Anti-Aldolase B antibody [5E2AD2] (ab129728)Immunoprecipitation image of ab129728. ALDOB antibody immunocaptured 39.4 kDa ALDOB from human liver homogenate (HLH), Rat liver homogenate (RLH) and Mouse liver homogenate (MLH). Gel stained with Coomassie brilliant blue G.
Lane 1: Ladder Lane 2: Human liver homogenate lysate
Lane 3: Rat liver homogenate lysate Lane 4: Mouse liver homogenate lysate -
![Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Aldolase B antibody [5E2AD2] (ab129728)Immunocytochemistry image of ab129728 stained HepG2 cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with ab129728 at 5 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (red) AlexaFluor® 594 goat anti-mouse IgG (H+L) used at 1/1000 dilution for 1h. 1% BSA was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Antigen retrieval step is recommended for a better signal. The target protein locates to the cytoplasm. -
![Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)](https://www.abcam.com/ps/products/129/ab129728/Images/ALDOB-Primary-antibodies-ab129728-3.jpg)
Flow Cytometry - Anti-Aldolase B antibody [5E2AD2] (ab129728)Flow cytometry using ab129728. Hela cells were stained with 5 µg/mL ab129728 antibody (blue) or no primary antibody control (red) and analyzed by flow cytometry.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab129728 has not yet been referenced specifically in any publications.
