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RecombinantRabMAb

Recombinant Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)

Submit a review Submit a question References (3)
Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)
  • Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 405 Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker
  • Suitable for: Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker
    See all TOMM20 primary antibodies

  • Description

    Alexa Fluor® 405 Rabbit monoclonal [EPR15581-54] to TOMM20 - Mitochondrial Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 405. Ex: 402nm, Em: 421nm

  • Tested applications

    Suitable for: Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human TOMM20 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q15388

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Flow Cyt (intra): HeLa cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab210047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

The cellular localisation of this product has been verified in ICC/IF.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.

  • Sequence similarities

    Belongs to the Tom20 family.

  • Cellular localization

    Mitochondrion outer membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • KIAA0016 antibody
    • MAS20 antibody
    • MGC117367 antibody
    • Mitochondrial 20 kDa outer membrane protein antibody
    • Mitochondrial import receptor subunit TOM20 homolog antibody
    • MOM19 antibody
    • Outer mitochondrial membrane receptor Tom20 antibody
    • TOM20 antibody
    • TOM20_HUMAN antibody
    • TOMM20 antibody
    • Translocase of outer mitochondrial membrane 20 homolog (yeast) antibody
    • Translocase of outer mitochondrial membrane 20 homolog type II antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)
    Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)

    Overlay histogram showing HeLa cells stained with ab210047 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min, blocked with Image-iT® FX Signal Enhancer for 30 min at 22ºC and then blocked with 1x PBS / 10% normal goat serum for 1 hr at 22ºC. Cells were then incubated with the antibody (ab210047, 1/5000 dilution) for 30 min at 22ºC.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 405 (ab208150) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW violet laser (405nm) and 450/50 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)
    Alexa Fluor® 405 Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (ab210047)

References (3)

Publishing research using ab210047? Please let us know so that we can cite the reference in this datasheet.

ab210047 has been referenced in 3 publications.

  • Thompson EA  et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep 34:108863 (2021). PubMed: 33691089
  • Thompson EA  et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. medRxiv N/A:N/A (2020). PubMed: 32935120
  • Zhu M  et al. Mitochondria Released by Apoptotic Cell Death Initiate Innate Immune Responses. Immunohorizons 2:384-397 (2018). PubMed: 30847435

Customer reviews and Q&As

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