Recombinant Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)](https://www.abcam.com/ps/products/195/ab195013/Images/ab195013-267959-anti-cd34-antibody-ep373y-alexa-fluor-488-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP373Y] to CD34
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-CD34 antibody [EP373Y]
See all CD34 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EP373Y] to CD34 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Dog, Pig, African bush elephant
Does not react with:
Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (intra): HUVEC cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 1.15 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP373Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195013 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Entrez Gene: 12490 Mouse
- Omim: 142230 Human
- SwissProt: P28906 Human
- SwissProt: Q64314 Mouse
- Unigene: 374990 Human
- Unigene: 29798 Mouse
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)
Overlay histogram showing HUVEC cells stained with ab195013 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab195013, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in HUVEC cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)](https://www.abcam.com/ps/products/195/ab195013/Images/ab195013-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab195013 has been referenced in 4 publications.
- Qian Y et al. Autologous decellularized extracellular matrix promotes adipogenic differentiation of adipose derived stem cells in low serum culture system by regulating the ERK1/2-PPAR? pathway. Adipocyte 10:174-188 (2021). PubMed: 33825675
- Su X et al. Nanomorphological and mechanical reconstruction of mesenchymal stem cells during early apoptosis detected by atomic force microscopy. Biol Open 9:N/A (2020). PubMed: 32086253
- Duong BTV et al. A liquid biopsy for detecting circulating mesothelial precursor cells: A new biomarker for diagnosis and prognosis in mesothelioma. EBioMedicine 61:103031 (2020). PubMed: 33045471
- Xu C et al. A pathogen-derived effector modulates host glucose metabolism by arginine GlcNAcylation of HIF-1a protein. PLoS Pathog 14:e1007259 (2018). PubMed: 30125331