Recombinant Alexa Fluor® 488 Anti-CD4 antibody [SP35] (ab309931)
![Alexa Fluor® 488 Anti-CD4 antibody [SP35] (ab309931)](https://www.abcam.com/ps/products/309/ab309931/Images/ab309931-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 488 Anti-CD4 antibody [SP35] (ab309931)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [SP35] to CD4
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-CD4 antibody [SP35]
See all CD4 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [SP35] to CD4 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP35 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-CD4 antibody [SP35], prediluted (ab101530)
- Anti-CD4 antibody [SP35] - BSA and Azide free (ab238798)
- PE Anti-CD4 antibody [SP35] (ab305622)
- APC Anti-CD4 antibody [SP35] (ab305623)
- HRP Anti-CD4 antibody [SP35] (ab305624)
- AP Anti-CD4 antibody [SP35] (ab308987)
- Alexa Fluor® 555 Anti-CD4 antibody [SP35] (ab312287)
- Alexa Fluor® 568 Anti-CD4 antibody [SP35] (ab312782)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab309931 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
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| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
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Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
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Alexa Fluor® 488 Anti-CD4 antibody [SP35] (ab309931)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab309931 has not yet been referenced specifically in any publications.