Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)](https://www.abcam.com/ps/products/171/ab171449/Images/ab171449-473503-AlexaFluor488AntiCPT1Aantibody8F6AE9immunocytochemistryhelahuman.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [8F6AE9] to CPT1A
- Suitable for: ICC/IF, Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]
See all CPT1A primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [8F6AE9] to CPT1A -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
Does not react with:
Cow -
Immunogen
Recombinant fragment corresponding to Human CPT1A aa 450 to the C-terminus (C terminal).
Database link: P50416 -
Positive control
- Flow cytometry: HeLa cells, HAP1-WT cells. ICC/IF: HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab171449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation. -
Clonality
Monoclonal -
Clone number
8F6AE9 -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171449 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 5 µg/ml.
|
|
| Flow Cyt | (4) |
Use a concentration of 5 µg/ml.
ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
Flow Cyt
Use a concentration of 5 µg/ml. ab171465 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Tissue specificity
Strong expression in kidney and heart, and lower in liver and skeletal muscle. -
Pathway
Lipid metabolism; fatty acid beta-oxidation. -
Involvement in disease
Defects in CPT1A are the cause of carnitine palmitoyltransferase 1A deficiency (CPT1AD) [MIM:255120]; also known as CPT-I deficiency or CPT1A deficiency. CPT1AD is a rare autosomal recessive metabolic disorder of long-chain fatty acid oxidation characterized by severe episodes of hypoketotic hypoglycemia usually occurring after fasting or illness. Onset is in infancy or early childhood. -
Sequence similarities
Belongs to the carnitine/choline acetyltransferase family. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 1374 Human
- Entrez Gene: 12894 Mouse
- Entrez Gene: 25757 Rat
- Omim: 600528 Human
- SwissProt: P50416 Human
- SwissProt: P97742 Mouse
- SwissProt: P32198 Rat
- Unigene: 503043 Human
see all -
Alternative names
- Carnitine O palmitoyltransferase 1 liver isoform antibody
- Carnitine O palmitoyltransferase I antibody
- Carnitine O palmitoyltransferase I liver isoform antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)
ab171449 staining CPT1A in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab171449 at 1/1000 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)](https://www.abcam.com/ps/products/171/ab171449/Images/ab171449-310279-anti-cpt1a-antibody-8f6ae9-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)Overlay histogram showing HAP1 wildtype (green line) and HAP1-CPT1A knockout cells (red line) stained with ab171449. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab171449, 1µg/ml dilution) for 30 min at 22°C.
A rabbit monoclonal IgG isotype control antibody (ab199091) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CPT1A knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. -
![Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)](https://www.abcam.com/ps/products/171/ab171449/Images/ab171449-172145-anti-cpt1a-antibody-8f6ae9-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)Flow cytometric analysis of HeLa cells (4% paraformaldehyde-fixed; methanol-permeablized) labeling CPT1A with ab171449 at 5 µg/mL (green) compared with an isotype control ab170192 at 5 µg/mL (black). Isotype control was labeled with a Goat Anti-mouse Alexa Fluor® 488 secondary antibody prior to signal measurement on the FL-1 channel.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)](https://www.abcam.com/ps/products/171/ab171449/Images/ab171449-172144-anti-cpt1a-antibody-8f6ae9-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449)Immunofluoresecence analysis of HeLa cells (4% paraformaldehyde-fixed, methanol-permeablized) labeling CPT1A with ab171449 at 5µg/ml overnight. 100x magnification.
A) HeLa cells labeled with ab171449 at 5 µg/mL.
B) HeLa cells labeled with Anti-HSP60 (1/3000, ab46798), Secondary antibody used was goat anti-rabbit Dylight-594 (1/1000, ab96897).
C) Merged Image of A and B showing specificity of mitochondrial staining.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab171449 has been referenced in 8 publications.
- Thompson EA et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. Cell Rep 34:108863 (2021). PubMed: 33691089
- Kumar B et al. Exosomes-driven lipolysis and bone marrow niche remodeling supports leukemia expansion. Haematologica 106:1484-1488 (2020). PubMed: 33054109
- Sawyer BT et al. Targeting Fatty Acid Oxidation to Promote Anoikis and Inhibit Ovarian Cancer Progression. Mol Cancer Res 18:1088-1098 (2020). PubMed: 32198139
- Thompson EA et al. Metabolic programs define dysfunctional immune responses in severe COVID-19 patients. medRxiv N/A:N/A (2020). PubMed: 32935120
- Kurupati RK et al. Age-related changes in B cell metabolism. Aging (Albany NY) 11:4367-4381 (2019). Flow Cyt . PubMed: 31283526
- Felices M et al. Continuous treatment with IL-15 exhausts human NK cells via a metabolic defect. JCI Insight 3:N/A (2018). PubMed: 29415897
- Zhang Y et al. Enhancing CD8+ T Cell Fatty Acid Catabolism within a Metabolically Challenging Tumor Microenvironment Increases the Efficacy of Melanoma Immunotherapy. Cancer Cell 32:377-391.e9 (2017). PubMed: 28898698
- Nguyen HD et al. Metabolic reprogramming of alloantigen-activated T cells after hematopoietic cell transplantation. J Clin Invest 126:1337-52 (2016). PubMed: 26950421
