Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4]
See all Cyclophilin F primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant full length protein corresponding to Rat Cyclophilin F.
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Positive control
- HDFn and HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading...
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Purification notes
ab156032 is a conjugated form of ab110324 which was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
E11AE12BD4 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 µg/ml.
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Flow Cyt |
Use a concentration of 0.2 µg/ml.
ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use a concentration of 5 µg/ml. |
Flow Cyt
Use a concentration of 0.2 µg/ml. ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 414346 Cow
- Entrez Gene: 10105 Human
- Entrez Gene: 105675 Mouse
- Entrez Gene: 282819 Rat
- Omim: 604486 Human
- SwissProt: P30404 Cow
- SwissProt: P30405 Human
- SwissProt: Q99KR7 Mouse
see all -
Form
This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein. -
Alternative names
- Cyclophilin 3 antibody
- cyclophilin D antibody
- Cyclophilin F antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml, (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
All blocking and antibodies were diluted in 10% normal goat serum. -
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)Immunofluorescent analysis of paraformaldehyde-fixed HDFn cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
All blocking and antibodies were diluted in 10% normal goat serum -
Flow cytometric analysis of HeLa cells labeling Cyclophilin F with ab156032 at 0.2 µg/ml (red) or an equal amount of IgG1 Alexa-488 Isotype control antibody (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab156032 has not yet been referenced specifically in any publications.