For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/alexa-fluor-488-cyclophilin-f-antibody-e11ae12bd4-ab156032.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Mitochondrial
Share by email

Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
  • Flow Cytometry - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)

Key features and details

  • Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Mouse, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Unconjugated

You may also be interested in

Primary
Product image
Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
Primary
Product image
Anti-Cyclophilin F antibody [EPR11311-121] (ab231155)
Primary
Product image
Anti-Cyclophilin F antibody [EPR11311-121] - BSA and Azide free (ab231287)

View more associated products

Overview

  • Product name

    Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4]
    See all Cyclophilin F primary antibodies
  • Description

    Alexa Fluor® 488 Mouse monoclonal [E11AE12BD4] to Cyclophilin F
  • Host species

    Mouse
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant full length protein corresponding to Rat Cyclophilin F.

  • Positive control

    • HDFn and HeLa cells.
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS
  • Concentration information loading...
  • Purification notes

    ab156032 is a conjugated form of ab110324 which was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    E11AE12BD4
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Alternative Versions

    • Anti-Cyclophilin F antibody [E11AE12BD4] (ab110324)
  • Isotype control

    • AlexaFluor ® 488 Mouse IgG1 [MOPC-21] - Isotype Control (ab234075)
  • Recombinant Protein

    • Recombinant human Cyclophilin F protein (ab79186)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab156032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 0.2 µg/ml.

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use a concentration of 0.2 µg/ml.

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P30405 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 414346 Cow
    • Entrez Gene: 10105 Human
    • Entrez Gene: 105675 Mouse
    • Entrez Gene: 282819 Rat
    • Omim: 604486 Human
    • SwissProt: P30404 Cow
    • SwissProt: P30405 Human
    • SwissProt: Q99KR7 Mouse
    • SwissProt: P29117 Rat
    • Unigene: 381072 Human
    • Unigene: 41656 Mouse
    • Unigene: 2923 Rat
    see all
  • Form

    This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • Alternative names

    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CyP-D antibody
    • CyP-M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml, (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
    Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
    All blocking and antibodies were diluted in 10% normal goat serum.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
    Immunofluorescent analysis of paraformaldehyde-fixed HDFn cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
    Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
    All blocking and antibodies were diluted in 10% normal goat serum
  • Flow Cytometry - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
    Flow Cytometry - Alexa Fluor® 488 Anti-Cyclophilin F antibody [E11AE12BD4] (ab156032)
    Flow cytometric analysis of HeLa cells labeling Cyclophilin F with ab156032 at 0.2 µg/ml (red) or an equal amount of IgG1 Alexa-488 Isotype control antibody (blue).

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab156032? Please let us know so that we can cite the reference in this datasheet.

ab156032 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab156032.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2023 Abcam plc. All rights reserved.