Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)](https://www.abcam.com/ps/products/154/ab154476/Images/ab154476-160657-anti-cytochrome-c-antibody-37ba11-alexa-fluor-488-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [37BA11] to Cytochrome C
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11]
See all Cytochrome C primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [37BA11] to Cytochrome C -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Caenorhabditis elegans
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Immunogen
Full length native protein (purified) corresponding to Cow Cytochrome C.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab154476 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
37BA11 -
Isotype
IgG2a -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipases
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 0.2 µg/ml.
ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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Flow Cyt (Intra)
Use a concentration of 0.2 µg/ml. ab171464 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. -
Involvement in disease
Defects in CYCS are the cause of thrombocytopenia type 4 (THC4) [MIM:612004]; also known as autosomal dominant thrombocytopenia type 4. Thrombocytopenia is the presence of relatively few platelets in blood. THC4 is a non-syndromic form of thrombocytopenia. Clinical manifestations of thrombocytopenia are absent or mild. THC4 may be caused by dysregulated platelet formation. -
Sequence similarities
Belongs to the cytochrome c family. -
Post-translational
modificationsBinds 1 heme group per subunit. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 510767 Cow
- Entrez Gene: 54205 Human
- Entrez Gene: 13063 Mouse
- Entrez Gene: 25309 Rat
- Omim: 123970 Human
- SwissProt: P62894 Cow
- SwissProt: P99999 Human
- SwissProt: P62897 Mouse
see all -
Alternative names
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)Immunocytochemistry with HeLa cells (100x) were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green and DAPI in blue, as a nuclear stain.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)](https://www.abcam.com/ps/products/154/ab154476/Images/ab154476-160656-anti-cytochrome-c-antibody-37ba11-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476) -
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)](https://www.abcam.com/ps/products/154/ab154476/Images/ab154476-160655-anti-cytochrome-c-antibody-37ba11-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (ab154476)Flow Cytometry for Anti-Cytochrome C Alexa-488 antibody (ab154476) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL Cytochrome C Alexa-488 (red) stained cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154476 has been referenced in 3 publications.
- Zhou H et al. The oncolytic compound LTX-401 targets the Golgi apparatus. Cell Death Differ 23:2031-2041 (2016). PubMed: 27588704
- Tian XF et al. PACE4 regulates apoptosis in human pancreatic cancer Panc-1 cells via the mitochondrial signaling pathway. Mol Med Rep 14:5205-5210 (2016). PubMed: 27779720
- Zhou H et al. The oncolytic peptide LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization. Oncotarget 6:26599-614 (2015). PubMed: 26378049