Recombinant Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192467)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EP1628Y] to Cytokeratin 8
- Suitable for: ICC, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Product nameAlexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]
See all Cytokeratin 8 primary antibodies
DescriptionAlexa Fluor® 488 Rabbit monoclonal [EP1628Y] to Cytokeratin 8
ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm
Tested applicationsSuitable for: ICC, Flow Cyt (Intra)more details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human Cytokeratin 8 aa 300-400 (C terminal). The exact sequence is proprietary.
Database link: P05787
- ICC/IF: HeLa cells Flow Cyt (intra): HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 4.60 x 10 -10 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (ab192468)
- HRP Anti-Cytokeratin 8 antibody [EP1628Y] (ab193094)
- PE Anti-Cytokeratin 8 antibody [EP1628Y] (ab209297)
- Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (ab210139)
- Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free (ab217173)
- Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (ab53280)
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt (Intra)||
Flow Cyt (Intra)
FunctionTogether with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Tissue specificityObserved in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Involvement in diseaseCirrhosis
Sequence similaritiesBelongs to the intermediate filament family.
modificationsPhosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cellular localizationCytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
- Information by UniProt
- Entrez Gene: 3856 Human
- Entrez Gene: 16691 Mouse
- Omim: 148060 Human
- SwissProt: P05787 Human
- SwissProt: P11679 Mouse
- Unigene: 533782 Human
- Unigene: 708445 Human
- Unigene: 358618 Mouse
- CARD2 antibody
- CK 8 antibody
- CK-8 antibody
Immunofluorescence analysis of mouse myoepithelial cells labelling KRT8 (right) with ab192467 at 1/100 dilution. The tissue was fixed in 10% neutral buffered formalin overnight. Paraffin embedding and sectioning were performed by the Rodent Histopathology Core at Harvard Medical School. Scale bar, 10 μm.
Immunofluorescence analysis of mouse postate tumor samples labelling cytokeratin 8 (green) with ab192467 at 1/100 dilution. SYP was also stained using ab206870 (red). Cells were fixed with formalin and embedded in paraffin. Sections were blocked with PBS-Tween (0.1%) containing 5% of BSA. Primary conjugated antibodies were simultaneously incubated overnight at 4°C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 µm.
Immunofluorescence staining of cytokeratin 8 in HeLa cells using ab192467. The cells were fixed with 4% formaldehyde (10 min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Triton X-100 for 1hr. The cells were then incubated with ab192647 at a working dilution of 1 in 100 (shown in green) and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin) at 1 µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1hr with AlexaFluor® 594 Goat anti-mouse IgG (H&L - preadsorbed) (ab150120) at 2 μg/ml (shown in pseudo-color red).
Nuclear DNA was labeled in blue with DAPI.
This product gave a positive signal in 100% methanol (5 min) fixed HeLa cells under the same testing conditions.
Image was taken with a Confocal microscope (Leica-microsystems, TCS SP8)
Flow cytometry analysis of HeLa cells stained with ab192467. The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab192467, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabeled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunofluorescence staining in human mammary organoids of cytokeratin 8 using ab192467 (green), cytokeratin 14 using ab206100 (red), and actin (white). Cells were fixed with 4% paraformaldehyde for 20 minutes at room temperature and permeabilized with 0.5% Triton X-100 for 10 minutes at 4 °C. Primary antibodies incubated overnight at 4 °C. Nuclear DNA was labelled with DAPI (blue). Scale bar = 100 µm. Organoids were imaged by confocal microscopy.
Datasheets and documents
ab192467 has been referenced in 10 publications.
- Mohad V et al. Inactivation of Lats1 and Lats2 highlights the role of hippo pathway effector YAP in larynx and vocal fold epithelium morphogenesis. Dev Biol 473:33-49 (2021). PubMed: 33515576
- Singh S et al. Pan-cancer Drivers are Recurrent Transcriptional Regulatory Heterogeneities in Early-stage Luminal Breast Cancer. Cancer Res N/A:N/A (2021). PubMed: 33531373
- Sulsenti R et al. Repurposing of the Antiepileptic Drug Levetiracetam to Restrain Neuroendocrine Prostate Cancer and Inhibit Mast Cell Support to Adenocarcinoma. Front Immunol 12:622001 (2021). PubMed: 33737929
- Li CM et al. Aging-Associated Alterations in Mammary Epithelia and Stroma Revealed by Single-Cell RNA Sequencing. Cell Rep 33:108566 (2020). PubMed: 33378681
- Hu X et al. The H-Y Antigen in Embryonic Stem Cells Causes Rejection in Syngeneic Female Recipients. Stem Cells Dev 29:1179-1189 (2020). PubMed: 32723003
- Rosenbluth JM et al. Organoid cultures from normal and cancer-prone human breast tissues preserve complex epithelial lineages. Nat Commun 11:1711 (2020). ICC . PubMed: 32249764
- Mevel R et al. RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development. Elife 9:N/A (2020). PubMed: 33025905
- Deuse T et al. Hypoimmunogenic derivatives of induced pluripotent stem cells evade immune rejection in fully immunocompetent allogeneic recipients. Nat Biotechnol 37:252-258 (2019). IF ; Mouse . PubMed: 30778232
- Crowell PD et al. Expansion of Luminal Progenitor Cells in the Aging Mouse and Human Prostate. Cell Rep 28:1499-1510.e6 (2019). Flow Cyt ; Mouse . PubMed: 31390564
- Barros-Silva JD et al. Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer. Cell Rep 25:3504-3518.e6 (2018). IF ; Mouse . PubMed: 30566873