Alexa Fluor® 488 Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab200807)
Key features and details
- Alexa Fluor® 488 Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG
Overview
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Product name
Alexa Fluor® 488 Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody -
Description
Alexa Fluor® 488 Rabbit polyclonal to ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human ERK1 (phospho T185 + Y187). Synthetic phosphopeptide derived from the region of ERK1 that contains threonine 202 (corresponding to 185 in ERK2) and tyrosine 204 (corresponding to 187 in ERK2).
Database link: P27361 -
Positive control
- NIH3T3 cells (serum starved), treated with PDGF (50 ng/mL for 15 minutes).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 0.2% BSA, 99% PBS
PBS without Mg2+ and Ca2+. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab200807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
| Notes |
|---|
|
ICC/IF
1/50. |
Target
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Cellular localization
ERK2: Nucleus. -
Database links
- Entrez Gene: 26413 Mouse
- Entrez Gene: 26417 Mouse
- SwissProt: P63085 Mouse
- SwissProt: Q63844 Mouse
- Unigene: 196581 Mouse
- Unigene: 8385 Mouse
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) antibody (ab200807)
Immunofluorescence analysis of NIH3T3 cells labeling ERK1 + ERK2 (phospho T185 + Y187) using ab200807 at 1/50 dilution. NIH3T3 cells were serum starved and either left untreated (left image) or treated with PDGF (50 ng/mL for 15 minutes) (right image).
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab200807 has been referenced in 7 publications.
- Cai ZP et al. Coeloglossum viride var. bracteatum extract attenuates staurosporine induced neurotoxicity by restoring the FGF2-PI3K/Akt signaling axis and Dnmt3. Heliyon 7:e07503 (2021). PubMed: 34401557
- Li H & Chen C Quercetin Has Antimetastatic Effects on Gastric Cancer Cells via the Interruption of uPA/uPAR Function by Modulating NF-?b, PKC-d, ERK1/2, and AMPKa. Integr Cancer Ther 17:511-523 (2018). PubMed: 28627240
- Tang F et al. CT45A1 siRNA silencing suppresses the proliferation, metastasis and invasion of lung cancer cells by downregulating the ERK/CREB signaling pathway. Mol Med Rep 16:6708-6714 (2017). PubMed: 28901509
- Li L et al. Antitumor activity of 2-[(2E)-3,7-dimethyl-2,6-octadienyl]-6-methyl-2,5-cyclohexadiene-1,4-dione isolated from the aerial part of Atractylodes macrocephala in hepatocellular carcinoma. Mol Med Rep 16:6299-6305 (2017). PubMed: 28901513
- Xiao Y et al. TGF-ß/MAPK signaling mediates the effects of bone marrow mesenchymal stem cells on urinary control and interstitial cystitis after urinary bladder transplantation. Am J Transl Res 9:1193-1202 (2017). PubMed: 28386345
- Wang J et al. A novel long intergenic noncoding RNA indispensable for the cleavage of mouse two-cell embryos. EMBO Rep 17:1452-1470 (2016). PubMed: 27496889
- Guo J et al. Berberine Protects Human Umbilical Vein Endothelial Cells against LPS-Induced Apoptosis by Blocking JNK-Mediated Signaling. Evid Based Complement Alternat Med 2016:6983956 (2016). WB ; Human . PubMed: 27478481
