Recombinant Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)](https://www.abcam.com/ps/products/195/ab195359/Images/ab195359-235179-anti-glucose-transporter-glut1-antibody-epr3915-alexa-fluor-488-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915]
See all Glucose Transporter GLUT1 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR3915] to Glucose Transporter GLUT1 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Dissociation constant (KD)
KD = 7.70 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3915 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab115730)
- Alexa Fluor® 647 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195020)
- HRP Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195021)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] - Low endotoxin, Azide free (ab196357)
- Alexa Fluor® 594 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab206360)
- PE Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab209449)
- Alexa Fluor® 405 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab210438)
- Anti-Glucose Transporter GLUT1 antibody [EPR3915] - BSA and Azide free (ab252403)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab195359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
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| Flow Cyt (Intra) |
1/500.
ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/50. |
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Flow Cyt (Intra)
1/500. ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody. |
Target
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Function
Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. -
Tissue specificity
Expressed at variable levels in many human tissues. -
Involvement in disease
Defects in SLC2A1 are the cause of glucose transporter type 1 deficiency syndrome (GLUT1DS) [MIM:606777]; also known as blood-brain barrier glucose transport defect. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
Defects in SLC2A1 are the cause of dystonia type 18 (DYT18) [MIM:612126]. DYT18 is an exercise-induced paroxysmal dystonia/dyskinesia. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT18 is characterized by attacks of involuntary movements triggered by certain stimuli such as sudden movement or prolonged exercise. In some patients involuntary exertion-induced dystonic, choreoathetotic, and ballistic movements may be associated with macrocytic hemolytic anemia. -
Sequence similarities
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cell membrane. Melanosome. Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 6513 Human
- Entrez Gene: 20525 Mouse
- Entrez Gene: 24778 Rat
- Omim: 138140 Human
- SwissProt: P11166 Human
- SwissProt: P17809 Mouse
- SwissProt: P11167 Rat
- Unigene: 473721 Human
see all -
Alternative names
- Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
- CSE antibody
- DYT17 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)
ab195359 staining Glucose Transporter GLUT1 in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab195359 at a working dilution of 1 in 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)](https://www.abcam.com/ps/products/195/ab195359/Images/ab195359-239561-anti-glucose-transporter-glut1-antibody-epr3915-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)Overlay histogram showing HepG2 cells stained with ab195359 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab195359, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
This antibody gave a positive signal in HepG2 fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)](https://www.abcam.com/ps/products/195/ab195359/Images/ab195359-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Glucose Transporter GLUT1 antibody [EPR3915] (ab195359)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (7)
ab195359 has been referenced in 7 publications.
- Pierzynowska K et al. Alpha-Amylase Inhibits Cell Proliferation and Glucose Uptake in Human Neuroblastoma Cell Lines. Biomed Res Int 2022:4271358 (2022). PubMed: 35924266
- Zeng W et al. Propofol regulates activated macrophages metabolism through inhibition of ROS-mediated GLUT1 expression. Inflamm Res 70:473-481 (2021). PubMed: 33751130
- Kurniawan H et al. Glutathione Restricts Serine Metabolism to Preserve Regulatory T Cell Function. Cell Metab 31:920-936.e7 (2020). PubMed: 32213345
- Lauder SN et al. Enhanced antitumor immunity through sequential targeting of PI3Kδ and LAG3. J Immunother Cancer 8:N/A (2020). PubMed: 33093155
- Khiew SH et al. Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells. J Clin Invest 130:3453-3466 (2020). Flow Cyt . PubMed: 32452834
- Ahl PJ et al. Met-Flow, a strategy for single-cell metabolic analysis highlights dynamic changes in immune subpopulations. Commun Biol 3:305 (2020). Flow Cyt . PubMed: 32533056
- Kurupati RK et al. Age-related changes in B cell metabolism. Aging (Albany NY) 11:4367-4381 (2019). Flow Cyt ; Human . PubMed: 31283526