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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)
  • Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR5610] to Hemopexin
  • Suitable for: ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610]
    See all Hemopexin primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR5610] to Hemopexin

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): HepG2 cells. ICC/IF: HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/100.

This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.

  • Tissue specificity

    Expressed by the liver and secreted in plasma.

  • Sequence similarities

    Belongs to the hemopexin family.
    Contains 5 hemopexin-like domains.

  • Post-translational
    modifications

    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Beta 1B glycoprotein antibody
      • Beta-1B-glycoprotein antibody
      • FLJ56652 antibody
      • HEMO antibody
      • HEMO_HUMAN antibody
      • Hemopexin antibody
      • Hpx antibody
      • HX antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)

      ab197881 staining Hemopexin in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197881 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5 min)

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)

      Overlay histogram showing HepG2 cells stained with ab197881 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab197881, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This antibody gave a positive signal in HepG2 fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)
      Alexa Fluor® 488 Anti-Hemopexin antibody [EPR5610] (ab197881)

    References (0)

    Publishing research using ab197881? Please let us know so that we can cite the reference in this datasheet.

    ab197881 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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