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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)

Submit a review Submit a question References (1)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)
  • Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR8364(B)] to IMPDH2
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)]
    See all IMPDH2 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR8364(B)] to IMPDH2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt (Intra): HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
ICC/IF
1/250.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).
Notes
Flow Cyt (Intra)
1/500.
ICC/IF
1/250.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

Target

  • Function

    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.

  • Tissue specificity

    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

  • Pathway

    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.

  • Sequence similarities

    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.

  • Post-translational
    modifications

    The N-terminus is blocked.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • IMDH2_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 2 antibody
    • IMP dehydrogenase 2 antibody
    • IMP oxireductase 2 antibody
    • IMPD 2 antibody
    • IMPD2 antibody
    • IMPDH 2 antibody
    • IMPDH II antibody
    • IMPDH-II antibody
    • Impdh2 antibody
    • Inosine 5' monophosphate dehydrogenase 2 antibody
    • Inosine 5' phosphate dehydrogenase 2 antibody
    • Inosine monophosphate dehydrogenase type II antibody
    • Inosine-5''-monophosphate dehydrogenase 2 antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)
    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)
    Overlay histogram showing HeLa cells stained with ab200770 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab200770, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)

    ab200770 staining IMPDH2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200770 at a 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)
    Alexa Fluor® 488 Anti-IMPDH2 antibody [EPR8364(B)] (ab200770)

References (1)

Publishing research using ab200770? Please let us know so that we can cite the reference in this datasheet.

ab200770 has been referenced in 1 publication.

  • Duong-Ly KC  et al. T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly. J Cell Sci 131:N/A (2018). PubMed: 30154209

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