Recombinant Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)](https://www.abcam.com/ps/products/201/ab201532/Images/ab201532-257039-anti-lamin-b-receptor-antibody-e398l-alexa-fluor-488-flow-cytometry.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [E398L] to Lamin B Receptor/LBR
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L]
See all Lamin B Receptor/LBR primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [E398L] to Lamin B Receptor/LBR -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E398L -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201349)
- Anti-Lamin B Receptor/LBR antibody [E398L] - BSA and Azide free (ab222391)
- APC Anti-Lamin B Receptor/LBR antibody [E398L] (ab224950)
- PE Anti-Lamin B Receptor/LBR antibody [E398L] (ab224951)
- Anti-Lamin B Receptor/LBR antibody [E398L] (ab32535)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
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| Flow Cyt (Intra) |
1/500.
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| Notes |
|---|
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ICC/IF
1/100. This product gave a positive signal in HepG2 cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min). |
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Flow Cyt (Intra)
1/500. |
Target
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Function
Anchors the lamina and the heterochromatin to the inner nuclear membrane. -
Involvement in disease
Defects in LBR are a cause of Pelger-Huet anomaly (PHA) [MIM:169400]. PHA is an autosomal dominant inherited abnormality of neutrophils, characterized by reduced nuclear segmentation and an apparently looser chromatin structure. Heterozygotes show hypolobulated neutrophil nuclei with coarse chromatin. Presumed homozygous individuals have ovoid neutrophil nuclei, as well as varying degrees of developmental delay, epilepsy, and skeletal abnormalities.
Defects in LBR are the cause of hydrops-ectopic calcification-moth-eaten skeletal dysplasia (HEM) [MIM:215140]; also known as Greenberg skeletal dysplasia. HEM is a rare autosomal recessive chondrodystrophy characterized by early in utero lethality and, therefore, considered to be nonviable. Affected fetuses typically present with fetal hydrops, short-limbed dwarfism, and a marked disorganization of chondro-osseous calcification and may present with polydactyly and additional nonskeletal malformations.
Defects in LBR may be a cause of Reynolds syndrome (REYNS) [MIM:613471]. It is a syndrome specifically associating limited cutaneous systemic sclerosis and primary biliray cirrhosis. It is characterized by liver disease, telangiectasia, abrupt onset of digital paleness or cyanosis in response to cold exposure or stress (Raynaud phenomenon), and variable features of scleroderma. The liver disease is characterized by pruritis, jaundice, hepatomegaly, increased serum alkaline phosphatase and positive serum mitochondrial autoantibodies, all consistent with primary biliary cirrhosis. -
Sequence similarities
Belongs to the ERG4/ERG24 family. -
Post-translational
modificationsPhosphorylated by CDK1 protein kinase in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Phosphorylation of LBR and HP1 proteins may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. -
Cellular localization
Nucleus inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 3930 Human
- Omim: 600024 Human
- SwissProt: Q14739 Human
- Unigene: 435166 Human
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Alternative names
- DHCR 14B antibody
- DHCR14B antibody
- Integral nuclear envelope inner membrane protein antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)Overlay histogram showing HepG2 cells stained with ab201532 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab201532, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)](https://www.abcam.com/ps/products/201/ab201532/Images/ab201532-243918-anti-lamin-b-receptor-antibody-e398l-alexa-fluor-488-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)ab201532 staining Lamin B Receptor/LBR in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201532 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 4% formaldehyde (10 min).
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![Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)](https://www.abcam.com/ps/products/201/ab201532/Images/ab201532-4-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 488 Anti-Lamin B Receptor/LBR antibody [E398L] (ab201532)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab201532 has not yet been referenced specifically in any publications.