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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
  • Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Envelope Marker
  • Suitable for: ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker
    See all Lamin B1 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EPR8985(B)] to Lamin B1 - Nuclear Envelope Marker

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells and HAP1 cells. IHC-P : human bladder transitional cell carcinoma tissue, mouse cerebrum cortex tissue and rat cerebrum tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
ICC/IF
1/100.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

  • Involvement in disease

    Defects in LMNB1 are the cause of leukodystrophy demyelinating autosomal dominant adult-onset (ADLD) [MIM:169500]. ADLD is a slowly progressive and fatal demyelinating leukodystrophy, presenting in the fourth or fifth decade of life. Clinically characterized by early autonomic abnormalities, pyramidal and cerebellar dysfunction, and symmetric demyelination of the CNS. It differs from multiple sclerosis and other demyelinating disorders in that neuropathology shows preservation of oligodendroglia in the presence of subtotal demyelination and lack of astrogliosis.

  • Sequence similarities

    Belongs to the intermediate filament family.

  • Post-translational
    modifications

    B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

  • Cellular localization

    Nucleus inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • ADLD antibody
    • lamin B1 antibody
    • Lamin-B1 antibody
    • LMN antibody
    • LMN2 antibody
    • LMNB antibody
    • Lmnb1 antibody
    • LMNB1_HUMAN antibody
    • MGC111419 antibody
    • OTTHUMP00000159218 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

    Immunohistochemistry analysis of paraffin-embedded rat cerebrum tissue sections labelling Lamin B1 with ab194106 at 1/100 dilution. The section was incubated with ab194106 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
    Nuclear envelope staining on rat cerebrum tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Negative control : The negative control is PBS.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

    ab194106 staining Lamin B1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194106 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/250 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

    Immunohistochemistry analysis of paraffin-embedded mouse cerebrum cortex tissue sections labelling Lamin B1 with ab194106 at 1/100 dilution. The section was incubated with ab194106 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
    Nuclear envelope staining on mouse cerebrum cortex tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Negative control : The negative control is PBS.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

    Immunohistochemistry analysis of paraffin-embedded human bladder transitional cell carcinoma tissue sections labelling Lamin B1 with ab194106 at 1/100 dilution. The section was incubated with ab194106 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 40 mins.
    Nuclear envelope staining on human bladder transitional cell carcinoma tissue. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Negative control : The negative control is PBS.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

    ab194106 staining Lamin B1 in wild-type HAP1 cells (top panel) and Lamin B1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Tween for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194106 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/250 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)
    Alexa Fluor® 488 Anti-Lamin B1 antibody [EPR8985(B)] - Nuclear Envelope Marker (ab194106)

References (2)

Publishing research using ab194106? Please let us know so that we can cite the reference in this datasheet.

ab194106 has been referenced in 2 publications.

  • Burlingame EA  et al. Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms. Cell Rep Methods 1:N/A (2021). PubMed: 34485971
  • Lin JR  et al. Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes. Elife 7:N/A (2018). PubMed: 29993362

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