Alexa Fluor® 488 Anti-LAMP2 antibody [H4B4] (ab187607)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LAMP2 antibody [H4B4] (ab187607)](https://www.abcam.com/ps/products/187/ab187607/Images/ab187607-1-anti-cd107b-lamp2-antibody-h4b4-flow-cytometry-peripheral-blood-human.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LAMP2 antibody [H4B4] (ab187607)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [H4B4] to LAMP2
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-LAMP2 antibody [H4B4]
See all LAMP2 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [H4B4] to LAMP2 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Specificity
ab187607 recognizes LAMP-2, an extensively glycosylated 100-120 kDa widely expressed lysosome-associated protein. -
Tested applications
Suitable for: Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Tissue, cells or virus corresponding to Human LAMP2. Human peripheral blood mononucleated cells (PBMC).
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Positive control
- Flow Cyt (intra): Human blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 0.2% BSA, 99% PBS -
Concentration information loading... -
Purity
Size exclusion -
Purification notes
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. It is then purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
H4B4 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
4 μl reagent / 100 μl of whole blood or 106 cells in a suspension |
| Notes |
|---|
|
Flow Cyt (Intra)
Use at an assay dependent concentration. 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension |
Target
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Function
Implicated in tumor cell metastasis. May function in protection of the lysosomal membrane from autodigestion, maintenance of the acidic environment of the lysosome, adhesion when expressed on the cell surface (plasma membrane), and inter-and intracellular signal transduction. Protects cells from the toxic effects of methylating mutagens. -
Tissue specificity
Isoform LAMP-2A is highly expressed in placenta, lung and liver, less in kidney and pancreas, low in brain and skeletal muscle. Isoform LAMP-2B is highly expressed in skeletal muscle, less in brain, placenta, lung, kidney and pancreas, very low in liver. -
Involvement in disease
Defects in LAMP2 are the cause of Danon disease (DAND) [MIM:300257]; also known as glycogen storage disease type 2B (GSD2B). DAND is a lysosomal glycogen storage disease characterized by the clinical triad of cardiomyopathy, vacuolar myopathy and mental retardation. It is often associated with an accumulation of glycogen in muscle and lysosomes. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsO- and N-glycosylated; some of the 16 N-linked glycans are polylactosaminoglycans. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. This protein shuttles between lysosomes, endosomes, and the plasma membrane. - Information by UniProt
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Database links
- Entrez Gene: 3920 Human
- Omim: 309060 Human
- SwissProt: P13473 Human
- Unigene: 496684 Human
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Form
Alternative splicing produces 3 isoforms. -
Alternative names
- CD107 antigen like family member B antibody
- CD107 antigen-like family member B antibody
- CD107b antibody
see all
Images
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Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-LAMP2 antibody [H4B4] (ab187607)Flow cytometry analysis (surface staining) of human peripheral blood labelingCD107b with ab187607.
Datasheets and documents
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Datasheet download
References (0)
ab187607 has not yet been referenced specifically in any publications.
