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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)

Submit a review Submit a question References (3)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)
  • Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1808Y] to Nrf2
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 Alexa Fluor® 647 APC Carrier Free HRP PE Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y]
    See all Nrf2 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1808Y] to Nrf2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab167152)

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.
Notes
Flow Cyt (Intra)
1/500.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control with this antibody.
ICC/IF
1/100.

Target

  • Function

    Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.

  • Tissue specificity

    Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle.

  • Sequence similarities

    Belongs to the bZIP family. CNC subfamily.
    Contains 1 bZIP domain.

  • Domain

    Acidic activation domain in the N-terminus, and DNA binding domain in the C-terminus.

  • Post-translational
    modifications

    Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus.

  • Cellular localization

    Cytoplasm > cytosol. Nucleus. Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents.
  • Information by UniProt

  • Database links

    • Alternative names

      • erythroid derived 2 antibody
      • HEBP1 antibody
      • like 2 antibody
      • NF E2 related factor 2 antibody
      • NF-E2-related factor 2 antibody
      • NF2L2_HUMAN antibody
      • NFE2 related factor 2 antibody
      • NFE2-related factor 2 antibody
      • Nfe2l2 antibody
      • Nrf 2 antibody
      • NRF2 antibody
      • Nuclear factor (erythroid derived 2) like 2 antibody
      • Nuclear factor antibody
      • nuclear factor erythroid 2 like 2 antibody
      • Nuclear factor erythroid 2 related factor 2 antibody
      • Nuclear factor erythroid 2-related factor 2 antibody
      • Nuclear factor erythroid derived 2 like 2 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)

      Overlay histogram showing HeLa cells stained with ab194984 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab194984, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

      Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)

      ab194984 staining Nrf2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab194984 at a working dilution of 1/100 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)
      Alexa Fluor® 488 Anti-Nrf2 antibody [EP1808Y] (ab194984)

    References (3)

    Publishing research using ab194984? Please let us know so that we can cite the reference in this datasheet.

    ab194984 has been referenced in 3 publications.

    • van Bruggen JAC  et al. Depletion of CLL cells by venetoclax treatment reverses oxidative stress and impaired glycolysis in CD4 T cells. Blood Adv 6:4185-4195 (2022). PubMed: 35580333
    • Pacheco MT  et al. Protective effect of sucupira oil nanoemulsion against oxidative stress in UVA-irradiated HaCaT cells. J Pharm Pharmacol 71:1532-1543 (2019). PubMed: 31378977
    • Kostyuk SV  et al. Changes of KEAP1/NRF2 and IKB/NF-?B Expression Levels Induced by Cell-Free DNA in Different Cell Types. Oxid Med Cell Longev 2018:1052413 (2018). PubMed: 29743966

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