Recombinant Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)](https://www.abcam.com/ps/products/308/ab308600/Images/ab308600-1-anti-pan-trk-antibody-epr17341-immunohistochemistry-mouse-cerebrum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR17341] to Pan Trk
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341]
See all Pan Trk primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR17341] to Pan Trk -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Mouse and rat cerebrum tissue.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17341 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- HRP Anti-Pan Trk antibody [EPR17341] (ab209465)
- Anti-Pan Trk antibody [EPR17341] - BSA and Azide free (ab218577)
- Biotin Anti-Pan Trk antibody [EPR17341] (ab252166)
- Alexa Fluor® 647 Anti-Pan Trk antibody [EPR17341] (ab311129)
- Alexa Fluor® 594 Anti-Pan Trk antibody [EPR17341] (ab311762)
- Alexa Fluor® 568 Anti-Pan Trk antibody [EPR17341] (ab313042)
- Alexa Fluor® 555 Anti-Pan Trk antibody [EPR17341] (ab313243)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Relevance
Family of neurotrophic tyrosine kinase (NTRK1/2/3) genes which encode TrkA, TrkB and TrkC protein kinases. The three family members are activated by different neurotrophins: TrkA is activated by Nerve growth factor (NGF), TrkB by Brain-derived neurotrophic factor (BDNF) or neurotrophin-4 (NT-4) and TrkC by NT-3. Neurotrophin signalling activates cellular pathways involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. Localization TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NT-3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. TrkC: Membrane. -
Database links
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 18213 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 29613 Rat
- Entrez Gene: 59109 Rat
- SwissProt: P15209 Mouse
- SwissProt: Q3UFB7 Mouse
see all -
Alternative names
- BDNF/NT-3 growth factors receptor antibody
- EML4 antibody
- ETV6 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)
Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Pan Trk with ab308600 at 1/100 dilution (5.0 ug/ml).
Positive staining on mouse cerebrum.
The section was incubated with ab308600 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)](https://www.abcam.com/ps/products/308/ab308600/Images/ab308600-3-anti-pan-trk-antibody-epr17341-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Pan Trk with ab308600 at 1/100 dilution (5.0 ug/ml).
Positive staining on rat cerebrum.
The section was incubated with ab308600 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)](https://www.abcam.com/ps/products/308/ab308600/Images/ab308600-2-anti-pan-trk-antibody-epr17341-immunohistochemistry-mouse-skeletal-muscl.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Pan Trk with ab308600 at 1/100 dilution (5.0 ug/ml).
Negative control: no staining on mouse skeletal muscle.
The section was incubated with ab308600 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. -
![Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)](https://www.abcam.com/ps/products/308/ab308600/Images/ab308600-504128-BenefitsofRecombinantantibodies.jpg)
Alexa Fluor® 488 Anti-Pan Trk antibody [EPR17341] (ab308600)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab308600 has not yet been referenced specifically in any publications.