Recombinant Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab196376)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta
- Suitable for: ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] -
Description
Alexa Fluor® 488 Rabbit monoclonal [Y92] to PDGFR alpha + PDGFR beta -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: NIH3T3 cells. WB: N-GST tagged Human PDGF Receptor beta (aa557 to 1106) recombinant protein, N-GST tagged Human PDGF Receptor alpha (aa550 to 1089) recombinant protein.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y92 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- HRP Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab195515)
- Alexa Fluor® 594 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206872)
- Alexa Fluor® 405 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab206873)
- Anti-PDGFR alpha + PDGFR beta antibody [Y92] - Low endotoxin, Azide free (ab215978)
- PE Anti-PDGFR alpha + PDGFR beta antibody [Y92] (C-terminal) (ab307638)
- APC Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (ab314942)
- Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (ab32570)
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196376 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50.
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Notes |
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ICC/IF
1/50. |
Target
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Cellular localization
PDGFR alpha: Membrane. PDGFR beta: Membrane. -
Database links
- Entrez Gene: 18595 Mouse
- Entrez Gene: 18596 Mouse
- SwissProt: P05622 Mouse
- SwissProt: P26618 Mouse
- Unigene: 221403 Mouse
- Unigene: 4146 Mouse
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-PDGFR alpha + PDGFR beta antibody [Y92] (ab196376)
ab196376 staining PDGFR alpha + beta in NIH3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab196376 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 1/167 dilution overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196376 has not yet been referenced specifically in any publications.