Recombinant Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)
![Flow Cytometry - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)](https://www.abcam.com/ps/products/307/ab307397/Images/ab307397-2-anti-sall4-antibody-sp289-flow-cytometry-293t1.jpg "Flow Cytometry - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [SP289] to Sall4
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 488 Anti-Sall4 antibody [SP289]
See all Sall4 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [SP289] to Sall4 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- NCCIT (human pluripotent embryonic carcinoma epithelial cell) 293T (human embryonic kidney epithelial cell)
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP289 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab307397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
|
| Flow Cyt (Intra) |
1/5000.
|
| Notes |
|---|
|
ICC/IF
1/50. |
|
Flow Cyt (Intra)
1/5000. |
Target
-
Function
Probable transcription factor. -
Tissue specificity
Expressed in testis. -
Involvement in disease
Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype. -
Sequence similarities
Belongs to the sal C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 57167 Human
- Omim: 607343 Human
- SwissProt: Q9UJQ4 Human
- Unigene: 517113 Human
-
Alternative names
- AA407717 antibody
- AL022809 antibody
- AW536104 antibody
see all
Images
-
Flow Cytometry - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)
-
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell, Left) / NCCIT (human pluripotent embryonic carcinoma epithelial cell, Right) cells labelling Sall4 with ab307397 at 1/5000 dilution (0.01ug) (Red) (Red) compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A at 1/ was used as the secondary antibody. Negative control: 293T (PMID:16840789).
-
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)](https://www.abcam.com/ps/products/307/ab307397/Images/ab307397-1-anti-sall4-antibody-sp289-immunocytochemistry-nccit1.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397) -
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Sall4 antibody [SP289] (ab307397)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labelling Sall4 with ab307397 at 1/50 (10.0 ug/ml) dilution, followed by antibody at 1/None dilution (Green). Confocal image showing nuclear staining in NCCIT cells.Negative control: 293T (PMID:16840789)Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is at 1/None dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab307397 has not yet been referenced specifically in any publications.