Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)](https://www.abcam.com/ps/products/205/ab205846/Images/ab205846-265456-anti-scp3-antibody-cor-10g11-7-alexa-fluor-488-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [Cor 10G11/7] to SCP3
- Suitable for: IHC-Fr
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7]
See all SCP3 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [Cor 10G11/7] to SCP3 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Hamster, Zebrafish
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-Fr: mouse frozen testis tissue section.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
Cor 10G11/7 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab205846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
1/100.
This product gave a positive signal in mouse frozen testis tissue fixed with 10% formaldehyde (10 min). |
| Notes |
|---|
|
IHC-Fr
1/100. This product gave a positive signal in mouse frozen testis tissue fixed with 10% formaldehyde (10 min). |
Target
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Function
Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. -
Tissue specificity
Testis-specific. -
Involvement in disease
Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. -
Sequence similarities
Belongs to the XLR/SYCP3 family. -
Cellular localization
Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. - Information by UniProt
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Database links
- Entrez Gene: 20962 Mouse
- SwissProt: P70281 Mouse
- Unigene: 297977 Mouse
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Alternative names
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)
IHC image of SCP3 staining in a section of frozen normal mouse testis.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab205846 at 1/100 (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab205846 has been referenced in 4 publications.
- Hu M et al. PRC1-mediated epigenetic programming is required to generate the ovarian reserve. Nat Commun 13:4510 (2022). PubMed: 35948547
- Yeh YH et al. Isolation of Murine Spermatogenic Cells using a Violet-Excited Cell-Permeable DNA Binding Dye. J Vis Exp N/A:N/A (2021). PubMed: 33522502
- Sandhu S et al. Centrosome dysfunction associated with somatic expression of the synaptonemal complex protein TEX12. Commun Biol 4:1371 (2021). PubMed: 34880391
- Abe H et al. The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis. Curr Biol 30:408-420.e5 (2020). PubMed: 31902729