Recombinant Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)](https://www.abcam.com/ps/products/302/ab302974/Images/ab302974-3-anti-sirp-alpha-antibody-epr16264-immunocytochemistry-thp-1.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR16264] to SIRP alpha
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264]
See all SIRP alpha primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR16264] to SIRP alpha -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Does not react with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: THP-1 (human monocytic leukemia monocyte). Flow cyt. intr.: THP-1 (human monocytic leukemia monocyte).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16264 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/50.
|
|
| Flow Cyt (Intra) |
1/50.
|
| Notes |
|---|
|
ICC/IF
1/50. |
|
Flow Cyt (Intra)
1/50. |
Target
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Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labeling SIRP alpha with ab302974 at 1/50 dilution (10.0 µg/ml) (Green). Confocal image showing membranous staining in THP-1 cells. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 µg/ml) (Red). The Nuclear counterstain was DAPI (Blue).
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![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)](https://www.abcam.com/ps/products/302/ab302974/Images/ab302974-2-anti-sirp-alpha-antibody-epr16264-flow-cytometry-thp-1.jpg)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)Flow cytometric (Intracellular) analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling SIRP alpha with ab302974 at 1/50 dilution (1µg) (Red), compared with a Rabbit IgG monoclonal [EPR25A] - Isotype Control (Alexa Fluor® 488) (ab199091) (Black) and an unlabeled control (cells without incubation with primary antibody) (Blue).
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![Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)](https://www.abcam.com/ps/products/302/ab302974/Images/ab302974-1-Benefits-of-Recombinant-antibodies.jpg)
Alexa Fluor® 488 Anti-SIRP alpha antibody [EPR16264] (ab302974)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab302974 has not yet been referenced specifically in any publications.