Recombinant Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)
![Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)](https://www.abcam.com/ps/products/282/ab282185/Images/ab282185-2-anti-sox2-antibody-sp76-flow-cytometry-f9.jpg "Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [SP76] to SOX2
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
-
Product name
Alexa Fluor® 488 Anti-SOX2 antibody [SP76]
See all SOX2 primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [SP76] to SOX2 -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- ICC: F9 cells. Flow Cyt: F9 cell.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP76 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
|
| ICC/IF |
1/50.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
ICC/IF
1/50. |
Target
-
Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 20674 Mouse
- SwissProt: P48432 Mouse
- Unigene: 65396 Mouse
-
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
see all
Images
-
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SOX2 with ab282185 at 1/500 dilution (0.1ug / Red) compared with a Rabbit monoclonal isotype control - Alexa Fluor® 488 (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
-
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)](https://www.abcam.com/ps/products/282/ab282185/Images/ab282185-1-anti-sox2-antibody-sp76-immunocytochemistry-f9.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labelling SOX2 with ab282185 at 1/50 dilution (10.0 µg/ml / Green). Confocal image showing nuclear staining in F9 (mouse embryonal carcinoma epithelial cell) cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). -
![Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)](https://www.abcam.com/ps/products/282/ab282185/Images/ab282185-3-benefitsofrecombinantantibodies.jpg)
Alexa Fluor® 488 Anti-SOX2 antibody [SP76] (ab282185)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab282185 has not yet been referenced specifically in any publications.