Recombinant Alexa Fluor® 488 Anti-STUB1 / CHIP antibody [EPR4447] (ab309692)
![Alexa Fluor® 488 Anti-STUB1 / CHIP antibody [EPR4447] (ab309692)](https://www.abcam.com/ps/products/309/ab309692/Images/ab309692-1-MTOConjugatedRecombinantAntibodyBenefits.jpg "Alexa Fluor® 488 Anti-STUB1 / CHIP antibody [EPR4447] (ab309692)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 488 Rabbit monoclonal [EPR4447] to STUB1/CHIP
- Suitable for: Target binding affinity, Antibody labelling
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
Related conjugates and formulations
Overview
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Product name
Alexa Fluor® 488 Anti-STUB1 / CHIP antibody [EPR4447]
See all STUB1/CHIP primary antibodies -
Description
Alexa Fluor® 488 Rabbit monoclonal [EPR4447] to STUB1/CHIP -
Host species
Rabbit -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested applications
Suitable for: Target binding affinity, Antibody labellingmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This conjugated primary antibody is released using a quantitative quality control method that evaluates binding affinity post-conjugation and efficiency of antibody labeling.
For suitable applications and species reactivity, please refer to the unconjugated version of this clone. This conjugated antibody is eligible for Abtrial: learn more here.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4447 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-STUB1/CHIP antibody [EPR4447] - BSA and Azide free (ab238966)
- PE Anti-STUB1 / CHIP antibody [EPR4447] (ab303131)
- APC Anti-STUB1 / CHIP antibody [EPR4447] (ab303132)
- HRP Anti-STUB1 / CHIP antibody [EPR4447] (ab303133)
- AP Anti-STUB1 / CHIP antibody [EPR4447] (ab308734)
- Alexa Fluor® 555 Anti-STUB1 / CHIP antibody [EPR4447] (ab311978)
- Alexa Fluor® 568 Anti-STUB1 / CHIP antibody [EPR4447] (ab312452)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab309692 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Target binding affinity |
Use at an assay dependent concentration.
|
|
| Antibody labelling |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
Target binding affinity
Use at an assay dependent concentration. |
|
Antibody labelling
Use at an assay dependent concentration. |
Target
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Function
E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Ubiquitinates NOS1 in concert with Hsp70 and Hsp40. Modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. Mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation. Mediates polyubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair: catalyzes polyubiquitination by amplifying the HUWE1/ARF-BP1-dependent monoubiquitination and leading to POLB-degradation by the proteasome. Mediates polyubiquitination of CYP3A4. -
Tissue specificity
Highly expressed in skeletal muscle, heart, pancreas, brain and placenta. Detected in kidney, liver and lung. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 3 TPR repeats.
Contains 1 U-box domain. -
Domain
The TPR domain is essential for ubiquitination mediated by UBE2D1. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Auto-ubiquitinated; mediated by UBE2D1 and UBE2D2. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- Antigen NY CO 7 antibody
- Antigen NY-CO-7 antibody
- C terminus of Hsp70-interacting protein antibody
see all
Images
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Alexa Fluor® 488 Anti-STUB1 / CHIP antibody [EPR4447] (ab309692)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab309692 has not yet been referenced specifically in any publications.