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RecombinantRabMAb

Recombinant Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)

Reviews (1) Submit a question References (1)
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)
  • Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 488 Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1
  • Suitable for: Flow Cyt (Intra), ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm

Conjugates logo Related conjugates and formulations

Carrier Free HRP Unconjugated

Overview

  • Product name

    Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y]
    See all Superoxide Dismutase 1 primary antibodies

  • Description

    Alexa Fluor® 488 Rabbit monoclonal [EP1727Y] to Superoxide Dismutase 1

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab199733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Notes
Flow Cyt (Intra)
1/500.
ICC/IF
1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

  • Involvement in disease

    Defects in SOD1 are the cause of amyotrophic lateral sclerosis type 1 (ALS1) [MIM:105400]. ALS1 is a familial form of amyotrophic lateral sclerosis, a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of cases leading to familial forms.

  • Sequence similarities

    Belongs to the Cu-Zn superoxide dismutase family.

  • Post-translational
    modifications

    Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.
    The ditryptophan cross-link at Trp-33 is reponsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.

  • Cellular localization

    Cytoplasm. The pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.
  • Information by UniProt

  • Database links

    • Alternative names

      • ALS antibody
      • ALS1 antibody
      • Amyotrophic lateral sclerosis 1 adult antibody
      • Cu/Zn SOD antibody
      • Cu/Zn superoxide dismutase antibody
      • Epididymis secretory protein Li 44 antibody
      • HEL S 44 antibody
      • Homodimer antibody
      • hSod1 antibody
      • Indophenoloxidase A antibody
      • IPOA antibody
      • Mn superoxide dismutase antibody
      • SOD antibody
      • SOD soluble antibody
      • SOD1 antibody
      • SOD2 antibody
      • SODC antibody
      • SODC_HUMAN antibody
      • Superoxide dismutase [Cu-Zn] antibody
      • Superoxide dismutase 1 antibody
      • Superoxide dismutase 1 soluble antibody
      • Superoxide dismutase Cu Zn antibody
      • Superoxide dismutase cystolic antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)
      Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)
      Overlay histogram showing HeLa cells stained with ab199733 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab199733, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG [EPR25A] Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)
      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)

      ab199733 staining Superoxide Dismutase 1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

    • Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)
      Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y] (ab199733)

    References (1)

    Publishing research using ab199733? Please let us know so that we can cite the reference in this datasheet.

    ab199733 has been referenced in 1 publication.

    • Li X  et al. A new function of copper zinc superoxide dismutase: as a regulatory DNA-binding protein in gene expression in response to intracellular hydrogen peroxide. Nucleic Acids Res 47:5074-5085 (2019). PubMed: 31162603

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Flow Cytometry abreview for Alexa Fluor® 488 Anti-Superoxide Dismutase 1 antibody [EP1727Y]

     Below Average
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Mouse Cell (Thymus)
    Permeabilization
    Yes - eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set according to the manufacturer's instructions.
    Gating Strategy
    Single cells - Thymocytes - Live cells (FVD negative cells)
    Specification
    Thymus
    Preparation
    Cell harvesting/tissue preparation method: The organ was removed and macerated in PBS with the help of a cell strainer and a piston.
    Sample buffer: Cells were resuspended and counted in PBS prior to initiating permeabilization and labeling with the eBioscience™ Foxp3/Transcription Factor Staining Buffer Set according to the manufacturer's instructions.
    Fixation
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 07 2022

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